FIGURE

Figure 6.

ID

ZDB-FIG-250415-82

Publication

Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

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Figure 6.

Whole-mount in situ analysis of cmyb mRNA colocalization with HSPCs at 5 dpf.

Spinning disk confocal images obtained with a 5 dpf larva [Tg(runx1+23:eGFP) fish line] expressing eGFP in HSPCs under the control of the runx1+23 enhancer. Panels are constituted from images obtained either in the trunk region (left: AGM) or in the tail (right: CHT), either from maximum z-projections of z-stacks or from single z-sections with 2× magnification of regions in white boxes, highlighting hematopoietic clusters (dashed lines). RNAscope signals (magenta) colocalize in the majority with HSPCs (see also Figure 7 and Video 3 for 3D reconstitution). AGM: aorta gonad mesonephros; CHT: caudal hematopoietic tissue; HSPCs: hematopoietic stem and progenitor cells. Scale bars = 20 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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