FIGURE

Video 1.

ID

ZDB-FIG-250415-80

Publication

Torcq et al., 2025 - Single Molecule Fluorescence In Situ Hybridization Using RNAscope to Study Hematopoietic and Vascular Interactions in the Zebrafish Embryo and Larva

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Video 1.

RNAscope in situ cmyb expression analysis in the pronephros region at 5 dpf.

Spinning disk confocal images (z-stack) obtained from a 5 dpf Tg(kdrl:eGFP) larva. The eGFP is expressed in endothelial cells under the control of the vascular kdrl promotor. Z-stack shows the pronephros region; consecutive confocal z-sections are 0.5 μm interspaced. The cmyb RNAscope signals (magenta) are localized in hematopoietic stem and progenitor cells (HSPCs) accumulated in the peri-glomerular region, surrounded by vessels. Unique z-sections (52/240, 86/240, 186/240, and 222/240) and maximum z-projection are also shown in Figure 5. This z-stack is also reconstituted in 3D in Video 2.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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