ZFIN Image: Torcq et al., 2025, Figure 7.

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Figure Caption

Figure 7. Cell and RNAscope segmentation workflow with Imaris.

All data shown are extracted from a single caudal hematopoietic tissue (CHT) z-stack showing the expression of cmyb (RNAscope, spots in magenta) in hematopoietic cells expressing eGFP under the control of the runx1+23 enhancer (the same 5 dpf larva and images as in Figure 6). (A) File conversion with Imaris File Converter. (B) Imaris 3D visualization in surpass mode. (C) Step-by-step workflow of cell segmentation, as detailed in Data Analysis, Section B. 3D cells and RNAscope signal segmentation with Imaris – Cell Biologist package.

Acknowledgments

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