FIGURE

Fig. 3

ID

ZDB-FIG-250204-3

Publication

Xiao et al., 2025 - Hsf1 is essential for proteotoxic stress response in smyd1b-deficient embryos and fish survival under heat shock

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Fig. 3

The upregulation of heat shock protein gene expression is a shared stress response in fish embryos with muscle defects from hsp90α1 and unc45b knockdown. Hsp70 and cochaperone gene expression was analyzed in hsp90α1 and unc45b knockdown (KD) embryos by in situ hybridization at 24 hpf. Compared with the controls (A, D, G, J, M, P, and S), a muscle-specific upregulation of hsp70.1/2/l (B, C), hsp70.3 (E, F), hspa1b (H, I), hsc70 (K, L), hspa8b (N, O), hsph1 (Q, R), and dnaja (T, U) was detected in hspa90α1-KD (B, E, H, K, N, Q, T) and unc45-KD (C, F, I, L, O, R, U) zebrafish embryos at 24 hpf. All larvae are side view, with anterior left and dorsal up. Scale bar = 100 μm.

Expression Data

Genes:	dnaja hsc70 hsp70.3 hspa1b hspa8b hsph1
Fish:	WT + MO1-unc45b WT + MO4-hsp90aa1.1
Knockdown Reagents:	MO1-unc45b MO4-hsp90aa1.1
Anatomical Term:	skeletal muscle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-unc45b WT + MO4-hsp90aa1.1
Knockdown Reagents:	MO1-unc45b MO4-hsp90aa1.1
Observed In:	skeletal muscle
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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