FIGURE

Fig. 8

ID

ZDB-FIG-250204-8

Publication

Xiao et al., 2025 - Hsf1 is essential for proteotoxic stress response in smyd1b-deficient embryos and fish survival under heat shock

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Fig. 8

Hsf1 is required for the muscle-specific upregulation of hsp chaperone and cochaperone gene expression in smyd1b mutant embryos. Expression of three hsp70 genes (hsp70.1/2/l, hspa8b, and hsc70), hsp40, hsp90α1 and unc45b mRNAs was analyzed in the WT control (A–F), hsf1−/− mutants (G–L), smyd1b−/− mutants (M–R) and smyd1b−/−; hsf1−/− double-mutant (S–X) embryos. No significant difference is visible between the WT (A-F) and hsf1−/− mutant (G–L) embryos. In contrast, loss of smyd1b dramatically upregulated the hsp chaperone and cochaperone gene expression in muscle cells of zebrafish embryos at 24 hpf (M–R). However, this muscle-specific upregulation was blocked in the smyd1b−/−; hsf1−/− double-mutant embryos (S–X). Scale bar = 100 μm.

Expression Data

Genes:	dnaja hsc70 hspa8b unc45b
Fish:	WT hsf1^mb30/mb30 hsf1^mb31/mb31 hsf1^mb32/mb32 smyd1b^{sa15678/sa15678} smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30 smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31 smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32
Anatomical Term:	skeletal muscle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smyd1b^{sa15678/sa15678} smyd1b^{sa15678/sa15678}; hsf1^mb30/mb30 smyd1b^{sa15678/sa15678}; hsf1^mb31/mb31 smyd1b^{sa15678/sa15678}; hsf1^mb32/mb32
Observed In:	skeletal muscle
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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