FIGURE

FIGURE 4

ID

ZDB-FIG-250109-290

Publication

Ercanbrack et al., 2024 - Frataxin is essential for zebrafish embryogenesis and pronephros formation

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FIGURE 4

Further analysis of pronephros segment development in fxn-deficient zebrafish embryos confirms reductions in several cell populations. (A) WISH using the probes wt1b, trim35-30-201, kcnj1a.1, and gata3 which mark the podocytes, multiciliated cells (MCCs), distal early and distal late domains respectively. Fxn morphants displayed a significant reduction in podocytes, reduction in MCC number, and reduced distal domains results with this set of markers, independently recapitulating the results shown in Figure 3. Scale bars = 50 um. Each boxed lateral region in the embryos corresponds to the inset, which shows a dorsal view of that corresponding area, with the exception of the gata3 panels which show lateral views in the corresponding inset. (B) Unpaired t-tests demonstrating significantly affected pronephric domains in the fxn morphants. **p < 0.005, ***p < 0.0005, ****p < 0.0001.

Expression Data

Genes:	gata3 kcnj1a.1 trim35-30 wt1b
Fish:	WT WT + MO1-fxn
Knockdown Reagent:	MO1-fxn
Anatomical Terms:	podocyte pronephric distal early tubule pronephric distal late tubule pronephros multi-ciliated epithelial cell
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fxn
Knockdown Reagent:	MO1-fxn
Observed In:	podocyte pronephric distal early tubule pronephric distal late tubule pronephros multi-ciliated epithelial cell
Stage:	26+ somites

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol