FIGURE

FIGURE 3

ID

ZDB-FIG-250109-289

Publication

Ercanbrack et al., 2024 - Frataxin is essential for zebrafish embryogenesis and pronephros formation

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FIGURE 3

Pronephros segment development is significantly disrupted in fxn-deficient zebrafish embryos. (A) WISH experiments reveal that fxn is required for the proper formation of the podocytes, multiciliated cells (MCCs), distal early, and distal late tubule. The previously mentioned domains were visualized via the nphs1,cetn4, slc12a1, and slc12a3 probes, respectively. Formation of the proximal convoluted and straight tubules, as assessed by expression of slc20a1a and trpm7 revealed no significant differences between wild-type embryos and fxn morphants. Scale bars = 50 um. Each boxed lateral region in the embryos corresponds to the inset, which shows a dorsal view of that corresponding area for nphs1, slc20a1a, cetn2, and slc12a1 or a lateral view of the corresponding area for trpm7 and slc12a3. (B) Unpaired t-tests of the phenotypes revealed by WISH. ****p < 0.0001.

Expression Data

Genes:	cetn2 nphs1 slc12a1 slc12a3 slc20a1a trpm7
Fish:	WT WT + MO1-fxn
Knockdown Reagent:	MO1-fxn
Anatomical Terms:	podocyte pronephric distal early tubule pronephric distal late tubule pronephric proximal convoluted tubule pronephric proximal straight tubule pronephros multi-ciliated epithelial cell
Stage:	26+ somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-fxn
Knockdown Reagent:	MO1-fxn
Observed In:	podocyte pronephric distal early tubule pronephric distal late tubule pronephros multi-ciliated epithelial cell
Stage:	26+ somites

Phenotype Detail

Acknowledgments

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