FIGURE

Figure S6.

ID

ZDB-FIG-241021-33

Publication

El-Daher et al., 2024 - Microglia are essential for tissue contraction in wound closure after brain injury in zebrafish larvae

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Figure S6.

Microglia possess the molecular machinery to exert forces, and accumulation leads to increased GFAP detectability.

(A) Confocal micrograph of a microglial cell in the optic tectum, which expresses both mpeg1:GFP and beta-actin:utrophin-mCherry, illustrating that the F-actin filaments accumulate along the exterior membrane of the microglia after stab injury. (B) Microglial cell (mpeg1:GFP) immunofluorescently labelled for non-muscular myosin II, the target for blebbistatin. The cell shows notable accumulation of myosin II. (C) Horizontal section of the tectum at 12 h post-injury is shown, together with a density plot for GFP fluorescence. Astrocytic processes labelled by her4.3:GFP-F show increased fluorescence next to the accumulation of microglia in the tectal neuropil. The scale bar in (A, B) represents 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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