Figure 7.

Microglial action relies on lcp1.

(A) Expression of lcp1 and apoeb as detected by hybridisation chain reaction fluorescence in situ hybridisation in injured larvae at 6 hpi. The intensity profiles shown on the graph (red: lcp1; green: apoeb) were measured along the orange lines shown in the images. (B) Zoomed-in hybridisation chain reaction fluorescence in situ hybridisation images for lcp1a (green) and apoeb (red), showing an almost complete overlap of patterns. Arrows point to lcp1+/apoeb+ cells at the lesion site. (C) Representative images of wound closure in control and haCR-injected animals are shown; orange dashed lines: injury site; white dashed lines: wound delimitation in the PVZ. (D) Boxplot representation of the repair index in all haCR-injected animals (P from an unpaired t test). (E) Boxplot representation of the number of microglia in the optic tectum at 24 hpi in haCR-injected animals. All t tests were non-significant (P > 0.05). For all experiments, n = 4 for control-injected, and n = 10 for haCR-injected animals. All scale bars represent 50 μm.