PUBLICATION
Microglia are essential for tissue contraction in wound closure after brain injury in zebrafish larvae
- Authors
- El-Daher, F., Enos, S.J., Drake, L.K., Wehner, D., Westphal, M., Porter, N.J., Becker, C.G., Becker, T.
- ID
- ZDB-PUB-241018-19
- Date
- 2024
- Source
- Life science alliance 8(1): (Journal)
- Registered Authors
- Becker, Catherina G., Becker, Thomas, Drake, Louisa, El-Daher, François, Enos, Stephen, Wehner, Daniel, Westphal, Markus
- Keywords
- none
- MeSH Terms
-
- Animals
- Astrocytes/metabolism
- Brain/metabolism
- Brain Injuries*/etiology
- Brain Injuries*/metabolism
- Brain Injuries*/pathology
- Cell Movement
- Disease Models, Animal
- Larva*
- Membrane Glycoproteins
- Microfilament Proteins/genetics
- Microfilament Proteins/metabolism
- Microglia*/metabolism
- Wound Healing*/physiology
- Zebrafish*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 39419547 Full text @ Life Sci Alliance
Citation
El-Daher, F., Enos, S.J., Drake, L.K., Wehner, D., Westphal, M., Porter, N.J., Becker, C.G., Becker, T. (2024) Microglia are essential for tissue contraction in wound closure after brain injury in zebrafish larvae. Life science alliance. 8(1):.
Abstract
Wound closure after brain injury is crucial for tissue restoration but remains poorly understood at the tissue level. We investigated this process using in vivo observations of larval zebrafish brain injury. Our findings show that wound closure occurs within the first 24 h through global tissue contraction, as evidenced by live-imaging and drug inhibition studies. Microglia accumulate at the wound site before closure, and computational models suggest that their physical traction could drive this process. Depleting microglia genetically or pharmacologically impairs tissue repair. At the cellular level, live imaging reveals centripetal deformation of astrocytic processes contacted by migrating microglia. Laser severing of these contacts causes rapid retraction of microglial processes and slower retraction of astrocytic processes, indicating tension. Disrupting the lcp1 gene, which encodes the F-actin-stabilising protein L-plastin, in microglia results in failed wound closure. These findings support a mechanical role of microglia in wound contraction and suggest that targeting microglial mechanics could offer new strategies for treating traumatic brain injury.
Errata / Notes
This article is corrected by ZDB-PUB-241127-10.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping