Figure S6.

Figure S6. Microglia possess the molecular machinery to exert forces, and accumulation leads to increased GFAP detectability.

(A) Confocal micrograph of a microglial cell in the optic tectum, which expresses both mpeg1:GFP and beta-actin:utrophin-mCherry, illustrating that the F-actin filaments accumulate along the exterior membrane of the microglia after stab injury. (B) Microglial cell (mpeg1:GFP) immunofluorescently labelled for non-muscular myosin II, the target for blebbistatin. The cell shows notable accumulation of myosin II. (C) Horizontal section of the tectum at 12 h post-injury is shown, together with a density plot for GFP fluorescence. Astrocytic processes labelled by her4.3:GFP-F show increased fluorescence next to the accumulation of microglia in the tectal neuropil. The scale bar in (A, B) represents 10 μm.