FIGURE

Fig. 4

ID

ZDB-FIG-241014-9

Publication

Hu et al., 2024 - Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation

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Fig. 4

Gα13 is required for efficient apical constriction of endodermal cells. Whole-mount immunostaining was conducted to determine ZO-1 localization. (A-F′) Confocal images showing ZO-1 localization (magenta, white arrowheads) in pharyngeal endodermal cells (green, labelled by memGFP) in the indicated embryos at 10 ss (A-B″) and 20 ss (C-F′). (A-B″) Confocal images of xz planes at 10 ss. (C-D′) Single confocal z-plane in xy view. (E-F′) Confocal mages of xz planes, captured at the positions marked by the white arrows in C,D. White dashed lines outline the endoderm. D, dorsal; V, ventral. (G) The frequencies of ZO-1 localization in the apical, basal and lateral region of endodermal cells in xz planes at 10 ss. Data from all embryos (squares) and all xz images (gray circles) are superimposed, with the number of xz images and embryos indicated. Data are mean±s.e.m. ****P<0.0001 (unpaired, two-tailed Student's t-test). (H) Average relative intensity of ZO-1 expression across the lateral region of the endoderm in xy planes at 20 ss. The number of regions of images and embryos analyzed is indicated. Data are mean±s.e.m.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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