PUBLICATION
Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation
- Authors
- Hu, B., Pinzour, J., Patel, A., Rooney, F., Zerwic, A., Gao, Y., Nguyen, N.T., Xie, H., Ye, D., Lin, F.
- ID
- ZDB-PUB-240912-1
- Date
- 2024
- Source
- Development (Cambridge, England) 151(19): (Journal)
- Registered Authors
- Lin, Fang, Ye, Ding
- Keywords
- Convergence and extension, E-cadherin, Gα13, Pharyngeal endoderm, RhoA
- MeSH Terms
-
- Actomyosin/metabolism
- Animals
- Animals, Genetically Modified
- Cadherins*/genetics
- Cadherins*/metabolism
- Cell Polarity
- Embryo, Nonmammalian/metabolism
- Endoderm*/cytology
- Endoderm*/embryology
- Endoderm*/metabolism
- GTP-Binding Protein alpha Subunits, G12-G13*/genetics
- GTP-Binding Protein alpha Subunits, G12-G13*/metabolism
- Gene Expression Regulation, Developmental*
- Morphogenesis/genetics
- Pharynx*/embryology
- Pharynx*/metabolism
- Signal Transduction
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- Zebrafish Proteins*/genetics
- Zebrafish Proteins*/metabolism
- rhoA GTP-Binding Protein*/genetics
- rhoA GTP-Binding Protein*/metabolism
- PubMed
- 39258889 Full text @ Development
Citation
Hu, B., Pinzour, J., Patel, A., Rooney, F., Zerwic, A., Gao, Y., Nguyen, N.T., Xie, H., Ye, D., Lin, F. (2024) Gα13 controls pharyngeal endoderm convergence by regulating E-cadherin expression and RhoA activation. Development (Cambridge, England). 151(19):.
Abstract
Pharyngeal endoderm cells undergo convergence and extension (C&E), which is essential for endoderm pouch formation and craniofacial development. Our prior work implicates Gα13/RhoA-mediated signaling in regulating this process, but underlying mechanisms remain unclear. Here, we used endoderm-specific transgenic and Gα13 mutant zebrafish to demonstrate that Gα13 plays a crucial role in pharyngeal endoderm C&E by regulating RhoA activation and E-cadherin expression. We showed that during C&E, endodermal cells gradually establish stable cell-cell contacts, acquire apical-basal polarity, and undergo actomyosin-driven apical constriction, processes that require Gα13. Additionally, we found Gα13-deficient embryos exhibit reduced E-cadherin expression, partially contributing to endoderm C&E defects. Notably, interfering with RhoA function disrupts spatial actomyosin activation without affecting E-cadherin expression. Collectively, our findings identify critical cellular processes for pharyngeal endoderm C&E and reveal that Gα13 controls this through two independent pathways: modulating RhoA activation and regulating E-cadherin expression, thus unveiling intricate mechanisms governing pharyngeal endoderm morphogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping