The expression patterns of the AMPK and mTOR pathways in chemical treated 7-day-old larvae and 1-month-old F2 heterozygous LMNA transgenic zebrafish. (A,Aʹ) Muscle endurance in the 1-month-old LMNA(L35P) and LMNA(R453W) fish, as well as swimming speed in the 7-day-old larvae with or without chemical treatment. The expression levels of key genes related to the AMPK and mTOR pathways were assessed using qPCR in both 7-day-old larvae and 1-month-old LMNA transgenic zebrafish. LMNA(L35P): n = 5; LMNA(L35P) + l-carnitine: n = 8; LMNA(R453W); n = 6; LMNA(R453W) + creatine: n = 7. (B,Bʹ) Expression of ampk gene. (C,Cʹ) Expression of foxo1a gene. (D,Dʹ) Expression of ppargc1a gene. (E,Eʹ) Expression of mTOR gene. (F,Fʹ) Expression of eif4ebp3l gene. For larvae: n = 20; for adult: n = 10. Statistical analysis was performed using ordinary One-way ANOVA, comparing the expression levels to the no drug treatment group. The presented P-values have been appropriately adjusted, and the level of statistical significance is indicated by the P-value, where denoted as following *represents 0.01 < P ≤ 0.05, **represents 0.001 < P ≤ 0.01, ***represents 0.0001 < P ≤ 0.001, and ****represents P ≤ 0.0001.
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