The muscle endurance of 1-month-old F2 heterozygous LMNA transgenic zebrafish after treated with chemicals. (A) Muscle endurance of AB(WT): n = 8; LMNA(L35P): n = 5; LMNA(L35P) + l-carnitine: n = 8. (B) Muscle endurance of AB(WT): n = 8; LMNA(R453W); n = 6; LMNA(R453W) + tyrosine: n = 8; LMNA(R453W) + taurine: n = 6; LMNA(R453W) + creatine: n = 7. (C) Muscle endurance of AB(WT): n = 9; LMNA(A539V): n = 7, LMNA(A539V) + l-carnitine: n = 7. Adult fish were treated with chemical for 1-month. Each dot represents one fish. Statistical analysis was conducted using a One-way ANONVA with Dunnett’s test, comparing the treated groups to either AB(WT) or the no drug treatment group. The presented p-values have been appropriately adjusted, and the level of statistical significance is indicated as following *represents 0.01 < P ≤ 0.05, **represents 0.001 < P ≤ 0.01, and ****represents P ≤ 0.0001. (D) Ingenuity Pathway Analysis (IPA) network pathway analysis revealed dysregulated genes in LMNA(R453W) mutant transgenic fish, resulting in decreased levels of creatine showing in blue. TGFB1 transforming growth factor-beta1, CCR2 C–C chemokine receptor type 2, C3 complement component 3, EDNRB endothelial receptor type B, CASP1 Caspase 1.
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