Figure 3

sirt7-null zebrafish larvae (sirt7^{ihblqs7/ihblqs7}) are more tolerant to hypoxia.A, Western blot analysis of sirt7 expression in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf). B, quantitative real-time PCR (qPCR) analysis of phd3 mRNA in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). C, qPCR analysis of pai1 mRNA in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). D, qPCR analysis of cited2 mRNA in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). E, qPCR analysis of vegfaa mRNA in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). F, qPCR analysis of epoa mRNA in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). G, o-dianisidine staining of erythrocytes in sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (6 dpf) under normoxia (Nor) and hypoxia (Hyp). H, quantitation of erythrocytes in (G) as determined by normalizing the intensities of o-dianisidine-stained cells. I, photographs of sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). J, survival curve of sirt7-null mutant (sirt7^{ihblqs7/ihblqs7}) and the wildtype sibling (sirt7^+/+) (3 dpf) under normoxia (Nor) and hypoxia (Hyp). p Values were calculated by two-way ANOVA analysis (B, C, D, E, F, and H) or log-rank test (J); ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001; data based on one representative experiment performed in three biological replicates from at least three independent experiments (mean ± SD).