FIGURE

FIGURE 4

ID

ZDB-FIG-220730-37

Publication

Mazzolini et al., 2022 - Wasl is crucial to maintain microglial core activities during glioblastoma initiation stages

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FIGURE 4

The actin nucleation promoting factor wasla is less expressed in microglia from HRasV12⁺ larvae. (a) Heatmap of differentially expressed (DE) genes (FDR < 0.05, Fold Change > |2|) from microglia transcriptome comparisons between 5 dpf HRasV12⁻ and HRasV12⁺ larval brains (11 genes), belonging to zebrafish actin cytoskeleton KEGG pathways [Regulation of actin cytoskeleton, Focal adhesion, Adherens junction, Tight junction and mTOR signaling pathway]. The actin nucleation promoting factor wasla is the top ranked gene of the list. See also Table S3. (b) Dot plots of normalized transformed read counts of the representative WASP family genes. Black plots represent non‐DE genes whereas, red plots correspond to DE genes at 5 dpf between control and HRasV12⁺ conditions. (b) The means ± SD of three independent experiments are plotted. (c) Dot plots of normalized transformed read counts of WASL gene expression of monocyte‐derived macrophages (MDMs) and microglia in human non tumor and glioma IDH WT brain samples (Klemm et al., 2020)

Expression Data

Genes:	cldnj fgf2 fgf10a fgfr3 fzd3b gata4 gng12a itgb6 myhb wasla
Fish:	hzm5Et/hzm5Et; io006Tg/io006Tg
Anatomical Terms:	microglial cell microglial cell tight junction
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hzm5Et/hzm5Et; io006Tg/io006Tg
Observed In:	microglial cell microglial cell tight junction
Stage:	Day 5

Phenotype Detail

Acknowledgments

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