FIGURE

FIGURE 2

ID

ZDB-FIG-220730-35

Publication

Mazzolini et al., 2022 - Wasl is crucial to maintain microglial core activities during glioblastoma initiation stages

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FIGURE 2

Pre‐neoplastic cells promote microglia proliferation. (a) Confocal images of the microglial population (magenta) of 5 dpf HRasV12⁻ (top panel) and HRasV12⁺ (lower panel) larvae. Scale bar represents 100 μm. Quantifications revealed a higher number of microglia in HRasV12⁺ brains compared to HRasV12⁻ brains at 5 dpf. HRasV12⁻: n = 15; HRasV12⁺: n = 15; N = 3. Error bars represent mean ± SD. (b) To measure microglia proliferation, the number of 4C4⁺/EdU⁺ cells was measured within the microglial population of 5 dpf control and HRasV12⁺ larvae. Results are expressed as a percentage of total microglia. HRasV12⁻: n = 17; HRasV12⁺: n = 17; N = 3. Error bars represent mean ± SD. Error bars represent mean ± SD. Images were captured using a Zeiss LSM710 confocal microscope with a 20X/NA 0.8 objective. All images represent the maximum intensity projections of Z stacks

Expression Data

Antibody:	Ab-4C4
Fish:	hzm5Et/hzm5Et hzm5Et/hzm5Et; io006Tg/io006Tg
Anatomical Term:	microglial cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hzm5Et/hzm5Et; io006Tg/io006Tg
Observed In:	microglial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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