FIGURE

L

ID

ZDB-FIG-220131-579

Publication

Wu et al., 2021 - Sensing of mycobacterial arabinogalactan by galectin-9 exacerbates mycobacterial infection

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Interaction of AG with galectins

Coomassie blue staining of galectin‐1, galectin‐3, galectin‐7, galectin‐8, galectin‐9, galectin‐14, and galectin‐related protein (LGALSL) post–SDS–PAGE analysis.

Chemical structures and conformations of β‐galactofuranoside and β‐galactopyranoside.

C–I

SPR assay of interactions of AG with indicated galectins including galectin‐1 (C), galectin‐3 (D), galectin‐7 (E), galectin‐8 (F), galectin‐14 (G), LGASL (H), and a summary table of KD (I). Curve fittings to a 1:1 Langmuir‐binding model calculated with TraceDrawer are shown as smooth black lines. The binding affinity of galectin‐9 and CRD2 to AG is highlighted in (I) in red.

SPR assay of interactions of AG with CRD1 of galectin‐9.

Coomassie blue staining of galectin‐9(1–146) and CRD2 of galectin‐9 post–SDS–PAGE analysis.

SPR assay of interactions of AG with galectin‐9(1–146).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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