FIGURE

H

ID

ZDB-FIG-220131-576

Publication

Wu et al., 2021 - Sensing of mycobacterial arabinogalactan by galectin-9 exacerbates mycobacterial infection

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AG induces expression of MMPs

Scatter plots of differentially expressed genes in the mouse peritoneal macrophages stimulated with AG (1 μg/ml) for 24 h as identified by RNA‐seq analysis. The RNA from the peritoneal macrophages was pooled and subjected to RNA‐seq.

GO class of gene expressions in mouse peritoneal macrophages stimulated with AG (1 μg/ml) for 24 h as identified by RNA‐seq analysis.

KEGG class of gene expressions in mouse peritoneal macrophages stimulated with AG (1 μg/ml) for 24 h as identified by RNA‐seq analysis.

D, E

qPCR analysis of Mmps including Mmp9, Mmp10, and Mmp12 mRNA from THP‐1 cells stimulated with AG (1 μg/ml) for indicated times (D) or at indicated concentrations (μg/ml) for 48 h (E).

qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 mRNA from mouse peritoneal macrophages stimulated with AG at indicated concentrations (μg/ml) for 24 h.

qPCR analysis of Mmps including Mmp9, Mmp10, Mmp12, and Mmp13 from the lungs of mice at indicated concentrations (μg) for 3 days post‐intraperitoneal administration of AG.

qPCR analysis of Mmps including Mmp9, Mmp10, and Mmp12 from THP‐1 cells stimulated with AG (1 μg/ml) for 48 h left untreated or pretreated with AG aptamers AA932 or AA835 (0.5 μg/ml).

Data information: Data in (D–F, H) are means ± SD averaged from 3 independent experiments performed with technical triplicates, and each symbol represents the mean of technical triplicates. Data in (G) are means ± SD of indicated mice from 1 of n = 3 independent experiments, and each symbol represents data from 1 mouse. One‐way ANOVA followed by Dunnett's post hoc test were used for statistical analysis, respectively. ns, not significant; **P < 0.01; ***P < 0.001; ****P < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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