FIGURE

Fig. 3

ID

ZDB-FIG-211116-3

Publication

Ghaddar et al., 2021 - Deleterious Effects of Overfeeding on Brain Homeostasis and Plasticity in Adult Zebrafish

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Fig. 3

DIO impact on BBB leakage, cerebral oxidative stress, and neuroinflammation. (A) First row: dorsal view pictures of the brain from DIO model CTRL and DIO fish. Second row: ventral view pictures of the brain from DIO model CTRL and DIO fish (n?=?3?6 brains). Note the barely blue stained brain parenchyma of DIO-treated fish. Third column: positive control showing Evans blue extravasation in the injured telencephalon (arrows). In (A), hypothalamic vibratome section showing Evans blue staining (Red) extravasation in the parenchyma. (B) Graph showing dot-blot quantification of 4-HNE staining in the brains of CTRL and DIO fish (n?=?4). (C) Proinflammatory cytokines (il1?, il6, and tnf?) cerebral gene expression in CTRL and DIO conditions (n?=?3 pools of 2 brains). (D) Counting of ramified, ?intermediate,? and ameboid microglia in the ventral part of the telencephalon (Vv Vd region) in both CTRL and DIO fish (n?=?6). Bar graph: SEM. Student's t-test: *p?

Expression Data

Genes:	il1b il6 tnfa
Fish:	WT
Condition:	increased food availability
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	increased food availability
Observed In:	brain 4-hydroxynon-2-enal brain inflammatory response endothelial blood brain barrier microglial cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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