FIGURE

Fig. 2

ID

ZDB-FIG-201216-2

Publication

Vallese et al., 2020 - An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Functional characterization of the SPLICS<sub>S/L</sub>-P2A probes.

a, d, g, j Cartoon showing the short- and long-range contact sites measured by the indicated reporters. b, e, h, k Representative images of HeLa cells expressing the indicated SPLICS_S/L-P2A probes to identify the co-localization of GFP (488 nm) or YFP (514 nm) fluorescent dots with targeted organelles. Different markers are used to label organelles: red fluorescent protein (mCherry) targeted to PM, anti-KDEL antibody (ER), mtHSP60 (mitochondria) and anti-PMP70 antibody (PO). Confocal images were acquired at 405, 488, 514, and 588 nm excitation wavelength. Scale bar 25 µm. c, f, i, l Quantification of the indicated SPLICS_S/L-P2A (short and long) contacts in HeLa cells. The SPLICS dots were quantified from the 3D rendering of a complete Z-stack (see Supplementary Fig. 2), mean ± SEM. The data were obtained from three independent transfections. (****P ≤ 0.0001 unpaired two-tailed t-test). Source data are provided as a source data file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.