PUBLICATION
An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo
- Authors
- Vallese, F., Catoni, C., Cieri, D., Barazzuol, L., Ramirez, O., Calore, V., Bonora, M., Giamogante, F., Pinton, P., Brini, M., Calì, T.
- ID
- ZDB-PUB-201215-2
- Date
- 2020
- Source
- Nature communications 11: 6069 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Calcium/metabolism
- HeLa Cells
- Endoplasmic Reticulum/metabolism
- Rats, Sprague-Dawley
- Zebrafish/metabolism
- Humans
- Cytosol/metabolism
- Genes, Reporter*
- Organelles/metabolism*
- Neurons/metabolism
- Cell Membrane/metabolism
- Green Fluorescent Proteins/metabolism*
- PubMed
- 33247103 Full text @ Nat. Commun.
Citation
Vallese, F., Catoni, C., Cieri, D., Barazzuol, L., Ramirez, O., Calore, V., Bonora, M., Giamogante, F., Pinton, P., Brini, M., Calì, T. (2020) An expanded palette of improved SPLICS reporters detects multiple organelle contacts in vitro and in vivo. Nature communications. 11:6069.
Abstract
Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The lack of tools to finely image inter-organelle proximity hindered our understanding on how these subcellular communication hubs mediate and regulate cell homeostasis. We develop an improved and expanded palette of split-GFP-based contact site sensors (SPLICS) for the detection of single and multiple organelle contact sites within a scalable distance range. We demonstrate their flexibility under physiological conditions and in living organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping