ZFIN ID: ZDB-FIG-200921-2
Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish. Neuroscience Bulletin   36(9):1023-1034 Full text @ Neurosci. Bull.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 2

The expression of markers of cerebellar granule cell progenitors is impaired in arl13b-deficient embryos. AI’ Representative images of in situ hybridization illustrate that the three paralogues of atoh1 (atoh1a, 1b and 1c) are expressed in distinct populations of cerebellar granule cell progenitors. atoh1a is expressed in the URL and LRL. Similar expression patterns of atoh1a occur in wild-type embryos (A) and arl13b mutants (B) while its expression is absent from the oral dorsomedial URL (dashed box) in arl13b morphants (C) at 36 hpf and 48 hpf (A’C’). The expression patterns of atoh1b remained unaffected in arl13b mutants and morphants (DF’). The expression level of atoh1c was decreased in the URL in arl13b morphants (I and I’) (cb, cerebellum; URL, upper rhombic lip; LRL, lower rhombic lip). AI’, Scale bar 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
atoh1a AB standard conditions Prim-25 corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
AB + MO1-arl13b standard conditions Prim-25 Not Detected corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec Not Detected corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
arl13bhi459Tg/hi459Tg standard conditions Prim-25 corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
atoh1b AB standard conditions Prim-25 corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
AB + MO1-arl13b standard conditions Prim-25 corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
arl13bhi459Tg/hi459Tg standard conditions Prim-25 corpus cerebelli dorso-medial region ISH
Prim-25 corpus cerebelli ventro-lateral region ISH
Long-pec corpus cerebelli dorso-medial region ISH
Long-pec corpus cerebelli ventro-lateral region ISH
atoh1c AB standard conditions Pec-fin corpus cerebelli ISH
Day 4 corpus cerebelli ISH
AB + MO1-arl13b standard conditions Pec-fin corpus cerebelli ISH
Day 4 corpus cerebelli ISH
arl13bhi459Tg/hi459Tg standard conditions Pec-fin corpus cerebelli ISH
Day 4 corpus cerebelli ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-arl13b standard conditions Prim-25 corpus cerebelli dorso-medial region atoh1a expression absent, abnormal
Long-pec corpus cerebelli dorso-medial region atoh1a expression absent, abnormal
Pec-fin corpus cerebelli atoh1c expression decreased amount, abnormal
Day 4 corpus cerebelli atoh1c expression decreased amount, abnormal
arl13bhi459Tg/hi459Tg standard conditions Pec-fin corpus cerebelli atoh1c expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuroscience Bulletin for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neurosci. Bull.