ZFIN ID: ZDB-FIG-200921-4
Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish. Neuroscience Bulletin   36(9):1023-1034 Full text @ Neurosci. Bull.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 4

Disruption of arl13b reduces both precursor and differentiated cerebellar Purkinje cells. AI Representative images of in situ hybridization using anti-sense probes against ptf1a and roraa to label Purkinje precursors and differentiated cells, respectively. The dorsomedial clusters of precursor of Purkinje cells and differentiated Purkinje neurons are selectively reduced (arrows). JL’’ Immunostaining of mature Purkinje neurons using anti-parvalbumin antibody reveals that the dorsomedial Purkinje neurons are reduced in arl13b-deficient embryos. AI, Scale bar 100 μm. JL’’ Scale bar 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ptf1a AB standard conditions Long-pec Purkinje cell layer corpus cerebelli ISH
AB + MO1-arl13b standard conditions Long-pec Purkinje cell layer corpus cerebelli dorso-medial region ISH
arl13bhi459Tg/hi459Tg standard conditions Long-pec Purkinje cell layer corpus cerebelli dorso-medial region ISH
roraa AB standard conditions Protruding-mouth Purkinje cell ISH
Day 4 Purkinje cell ISH
AB + MO1-arl13b standard conditions Protruding-mouth Purkinje cell ISH
Day 4 Purkinje cell ISH
arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth Purkinje cell ISH
Day 4 Purkinje cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb IHC AB standard conditions Day 5 Purkinje cell layer corpus cerebelli Purkinje cell
IHC AB + MO1-arl13b standard conditions Day 5 Purkinje cell layer corpus cerebelli dorso-medial region
IHC Day 5 Purkinje cell layer corpus cerebelli Purkinje cell
IHC arl13bhi459Tg/hi459Tg standard conditions Day 5 Purkinje cell layer corpus cerebelli dorso-medial region
IHC Day 5 Purkinje cell layer corpus cerebelli Purkinje cell
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-arl13b standard conditions Long-pec cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Long-pec Purkinje cell layer corpus cerebelli dorso-medial region ptf1a expression decreased amount, abnormal
Protruding-mouth cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Protruding-mouth Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
Day 4 cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Day 4 Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
Day 5 cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Day 5 cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Day 5 Purkinje cell layer corpus cerebelli dorso-medial region ab1-pvalb labeling decreased amount, abnormal
Day 5 Purkinje cell layer corpus cerebelli dorso-medial region has fewer parts of type Purkinje cell, abnormal
Day 5 Purkinje cell layer corpus cerebelli dorso-medial region has fewer parts of type Purkinje cell, abnormal
arl13bhi459Tg/hi459Tg standard conditions Long-pec cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Long-pec Purkinje cell layer corpus cerebelli dorso-medial region ptf1a expression decreased amount, abnormal
Protruding-mouth cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Protruding-mouth Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
Day 4 cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Day 4 Purkinje cell layer corpus cerebelli has fewer parts of type Purkinje cell, abnormal
Day 5 cerebellar Purkinje cell differentiation decreased occurrence, abnormal
Day 5 Purkinje cell layer corpus cerebelli dorso-medial region ab1-pvalb labeling decreased amount, abnormal
Day 5 Purkinje cell layer corpus cerebelli dorso-medial region has fewer parts of type Purkinje cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuroscience Bulletin for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neurosci. Bull.