ZFIN ID: ZDB-FIG-200921-3
Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish. Neuroscience Bulletin   36(9):1023-1034 Full text @ Neurosci. Bull.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 3

Disruption of arl13b impairs the development of cerebellar granule cells. A Expression of the granule cell progenitor marker, zic1, in the cerebellum of wild-type embryos at 48 hpf revealed by in situ hybridization. B, C Expression of zic1 is reduced in the URL of both arl13b mutant and morphant embryos compared with wild-type embryos. Note that zic1 expression is severely reduced in the dorsomedial subregions of the URL (arrows). D The expression of the differentiated granule cell marker, reelin, in the cerebellum of wild-type embryos at 3 dpf. E, F Expression of reelin is reduced in the cerebellum of both arl13b mutant and morphant embryos. Note that in some embryos reelin expression is almost absent in the dorsal medial subregions of the cerebellum (colored ovals). GG’’ In Tg(neurod1:eGFP) transgenic embryos, GFP+ granule cells are grouped into three clusters, the dorsomedial (dashed ovals), dorsoposterior, and ventrolateral subdivisions. HH’’ The pattern of GFP+ granule cells is dramatically altered in the cerebellum, and particularly in the dorsomedial cerebellar subdivisions (dashed ovals) are severely affected in arl13b morphants. The parallel fibers connecting the two hemispheres are disrupted in arl13b morphants. Dorsal views of the embryos are shown. IL Malformations of the dorsomedial cerebellar subdivisions (dashed ovals) and parallel fibers are also present in arl13b mutants both at 3 dpf and 4 dpf. cb, cerebellum; PF, parallel fiber. AF, Scale bar 100 μm. GL, Scale bar 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP arl13bhi459Tg/hi459Tg; nl1Tg standard conditions Protruding-mouth corpus cerebelli dorso-medial region IHC
Day 4 corpus cerebelli dorso-medial region IHC
nl1Tg standard conditions Protruding-mouth corpus cerebelli dorso-medial region IHC
Day 4 corpus cerebelli dorso-medial region IHC
nl1Tg + MO1-arl13b standard conditions Day 4 corpus cerebelli dorso-medial region IHC
reln AB standard conditions Protruding-mouth corpus cerebelli dorso-medial region ISH
AB + MO1-arl13b standard conditions Protruding-mouth corpus cerebelli dorso-medial region ISH
arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth corpus cerebelli dorso-medial region ISH
zic1 AB standard conditions Long-pec corpus cerebelli dorso-medial region ISH
AB + MO1-arl13b standard conditions Long-pec corpus cerebelli dorso-medial region ISH
arl13bhi459Tg/hi459Tg standard conditions Long-pec corpus cerebelli dorso-medial region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-arl13b standard conditions Long-pec cerebellar granule cell differentiation decreased occurrence, abnormal
Long-pec corpus cerebelli dorso-medial region zic1 expression decreased amount, abnormal
Protruding-mouth cerebellar granule cell differentiation decreased occurrence, abnormal
Protruding-mouth corpus cerebelli dorso-medial region reln expression decreased amount, abnormal
arl13bhi459Tg/hi459Tg standard conditions Long-pec cerebellar granule cell differentiation decreased occurrence, abnormal
Long-pec corpus cerebelli dorso-medial region zic1 expression decreased amount, abnormal
Protruding-mouth cerebellar granule cell differentiation decreased occurrence, abnormal
Protruding-mouth corpus cerebelli dorso-medial region reln expression decreased amount, abnormal
arl13bhi459Tg/hi459Tg; nl1Tg standard conditions Protruding-mouth cerebellar granule cell differentiation decreased occurrence, abnormal
Protruding-mouth corpus cerebelli dorso-medial region EGFP expression decreased amount, abnormal
Protruding-mouth molecular layer corpus cerebelli parallel fiber disorganized, abnormal
Day 4 cerebellar granule cell differentiation decreased occurrence, abnormal
Day 4 corpus cerebelli dorso-medial region EGFP expression decreased amount, abnormal
Day 4 molecular layer corpus cerebelli parallel fiber disorganized, abnormal
nl1Tg + MO1-arl13b standard conditions Day 4 cerebellar granule cell differentiation decreased occurrence, abnormal
Day 4 corpus cerebelli dorso-medial region EGFP expression decreased amount, abnormal
Day 4 molecular layer corpus cerebelli parallel fiber disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuroscience Bulletin for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neurosci. Bull.