ZFIN ID: ZDB-FIG-200921-6
Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish. Neuroscience Bulletin   36(9):1023-1034 Full text @ Neurosci. Bull.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6

Treating the arl13b mutants with lithium mitigates the morphological defects in the cerebellum. AD Representative images of embryos treated with 50 mmol/L LiCl at 30–37 hpf, fixed at 3 dpf, and immunostained with anti-tubulin antibody to reveal cerebellar morphology. Treatment of wild-type embryos with Li+ does not affect the cerebellar morphology (A, B) (arrows). The morphological defects of the cerebellum in arl13b mutants treated with Li+ are partially rescued (C, D) (arrow). AD, Scale bar 100 μm. E Statistics revealing that the proportion of arl13b mutant embryos with cerebellar defects is dramatically decreased by LiCl treatment. FI’’ Representative images of Tg(neurod1:EGFP) transgenic embryos used to label granule cells. Treating wild-type transgenic embryos with Li+ causes no defect (FG’’) (dashed ovals). Treating arl13b morphant transgenic embryos restores the dorsomedial cluster of granule cells (HI’’) (dashed ovals). FI’’, Scale bar 100 μm. J The proportion of arl13b morphant embryos with cerebellar defects in the dorsomedial clusters is dramatically reduced by LiCl treatment.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nl1Tg standard conditions Protruding-mouth corpus cerebelli cerebellar granule cell IHC
Protruding-mouth corpus cerebelli dorso-medial region IHC
nl1Tg chemical treatment by environment: lithium chloride Protruding-mouth corpus cerebelli cerebellar granule cell IHC
Protruding-mouth corpus cerebelli dorso-medial region IHC
nl1Tg + MO1-arl13b standard conditions Protruding-mouth corpus cerebelli cerebellar granule cell IHC
Protruding-mouth corpus cerebelli dorso-medial region IHC
nl1Tg + MO1-arl13b chemical treatment by environment: lithium chloride Protruding-mouth corpus cerebelli cerebellar granule cell IHC
Protruding-mouth corpus cerebelli dorso-medial region IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB standard conditions Protruding-mouth corpus cerebelli parallel fiber
IHC AB chemical treatment by environment: lithium chloride Protruding-mouth corpus cerebelli parallel fiber
IHC arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth corpus cerebelli parallel fiber
IHC arl13bhi459Tg/hi459Tg chemical treatment by environment: lithium chloride Protruding-mouth corpus cerebelli parallel fiber
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: lithium chloride Protruding-mouth cerebellum morphogenesis normal process quality, normal
Protruding-mouth parallel fiber axon development normal process quality, normal
arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth cerebellum morphogenesis decreased process quality, abnormal
Protruding-mouth parallel fiber axon development decreased process quality, abnormal
arl13bhi459Tg/hi459Tg chemical treatment by environment: lithium chloride Protruding-mouth cerebellum morphogenesis process quality, ameliorated
Protruding-mouth parallel fiber axon development process quality, ameliorated
nl1Tg chemical treatment by environment: lithium chloride Protruding-mouth cerebellum morphogenesis normal process quality, normal
Protruding-mouth granular layer corpus cerebelli has normal numbers of parts of type cerebellar granule cell, normal
nl1Tg + MO1-arl13b standard conditions Protruding-mouth cerebellum morphogenesis decreased process quality, abnormal
Protruding-mouth corpus cerebelli dorso-medial region EGFP expression decreased amount, abnormal
Protruding-mouth granular layer corpus cerebelli has fewer parts of type cerebellar granule cell, abnormal
nl1Tg + MO1-arl13b chemical treatment by environment: lithium chloride Protruding-mouth cerebellum morphogenesis process quality, ameliorated
Protruding-mouth corpus cerebelli dorso-medial region EGFP expression amount, ameliorated
Protruding-mouth granular layer corpus cerebelli has number of cerebellar granule cell, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Neuroscience Bulletin for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neurosci. Bull.