ZFIN ID: ZDB-FIG-200921-1
Zhu et al., 2020 - The Joubert Syndrome Gene arl13b is Critical for Early Cerebellar Development in Zebrafish. Neuroscience Bulletin   36(9):1023-1034 Full text @ Neurosci. Bull.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 1

Knockdown of arl13b impairs posture, locomotion, and cerebellar morphology in zebrafish larvae. A Wild-type sibling larvae remain vertically oriented at 5 days post-fertilization (dpf), with both eyes visible from a top view (arrows). B In contrast, larvae injected with arl13b MO (subthreshold dose, 7.3 ng) often lie on their side at the bottom of the dish, with only one eye visible (arrows). Note that the body of the subthreshold-dose arl13b morphants are relatively straight and only slightly curved. C Statistics of the posture of zebrafish larvae at 5 dpf. D Wild-type larvae perform stereotyped spontaneous swimming with small bending angles. E The arl13b morphants (subthreshold dose, 7.3 ng) swim slower and exhibit greater bending angles. FH Immunostaining with acetylated tubulin antibody outlines the cerebellum of larvae at 3 dpf. Comparing the dorsal view of wild-type embryos (F) with arl13b mutants (G) reveals morphological defects of the cerebellum (arrows). The cerebellar defects were also present in embryos injected with arl13b MO (H) (cb, cerebellum). I Statistics of the embryos with morphological defects of the cerebellum. The number of embryos examined in each condition is indicated above each column. AE, Scale bar 1 cm. FH, Scale bar 100 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB standard conditions Protruding-mouth intertectal commissure
IHC Protruding-mouth parallel fiber
IHC AB + MO1-arl13b standard conditions Protruding-mouth intertectal commissure
IHC Protruding-mouth parallel fiber
IHC arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth intertectal commissure
IHC Protruding-mouth parallel fiber
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-arl13b standard conditions Protruding-mouth optic tectum axon development normal process quality, normal
Protruding-mouth parallel fiber axon development decreased process quality, abnormal
Day 5 swimming decreased process quality, abnormal
arl13bhi459Tg/hi459Tg standard conditions Protruding-mouth optic tectum axon development normal process quality, normal
Protruding-mouth parallel fiber axon development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Neuroscience Bulletin for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neurosci. Bull.