ZFIN ID: ZDB-FIG-200401-6
Kobayashi et al., 2019 - Dual role of Jam3b in early hematopoietic and vascular development. Development (Cambridge, England)   147(1) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: 26+ somites to Day 4

Fig. 1 Mutation of jam3b causes defects in both hematopoietic and vascular development. (A) Expression of runx1 and cmyb in the DA of WT or jam3bsa37 embryos. Magnification of boxed area shown underneath. (B) Representative image (left) and quantification (right) of cd41:GFP; kdrl:mCherry double-positive cells in WT or jam3bsa37 embryos. White arrowheads indicate double-positive cells in the ventral floor of the DA. Magnification of boxed area shown underneath. Data are mean±s.d. *P<0.00001 (unpaired two-tailed Student's t-test). (C) Expression of cmyb in the CHT and rag1 in the thymus of WT or jam3bsa37 embryos. (D) Expression of fli1a (endothelium), efnb2a (DA), and flt4 (PCV) in WT or jam3bsa37 embryos. (E) Expression of gata1a and hbae3 in primitive erythrocytes of WT or jam3bsa37 embryos. (F) Expression of fli1a and l-plastin (macrophage) in WT or jam3bsa37 embryos. Black arrowheads indicate the expression domain of each gene. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
efnb2a WT standard conditions Prim-5 dorsal aorta ISH
jam3bsa37/sa37 standard conditions Prim-5 dorsal aorta ISH
EGFP jam3bsa37/sa37; la2Tg; s896Tg standard conditions Long-pec hematopoietic multipotent progenitor cell IFL
Long-pec hematopoietic stem cell IFL
la2Tg; s896Tg standard conditions Long-pec hematopoietic multipotent progenitor cell IFL
Long-pec hematopoietic stem cell IFL
fli1 WT standard conditions 26+ somites dorsal aorta ISH
26+ somites posterior cardinal vein ISH
Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
jam3bsa37/sa37 standard conditions 26+ somites dorsal aorta ISH
26+ somites posterior cardinal vein ISH
Prim-5 dorsal aorta ISH
Prim-5 posterior cardinal vein ISH
flt4 WT standard conditions Prim-5 posterior cardinal vein ISH
jam3bsa37/sa37 standard conditions Prim-5 posterior cardinal vein ISH
gata1a WT standard conditions 26+ somites nucleate erythrocyte ISH
jam3bsa37/sa37 standard conditions 26+ somites nucleate erythrocyte ISH
hbae3 WT standard conditions 26+ somites nucleate erythrocyte ISH
jam3bsa37/sa37 standard conditions 26+ somites nucleate erythrocyte ISH
lcp1 WT standard conditions 26+ somites macrophage ISH
jam3bsa37/sa37 standard conditions 26+ somites macrophage ISH
mCherry jam3bsa37/sa37; la2Tg; s896Tg standard conditions Long-pec hematopoietic multipotent progenitor cell IFL
Long-pec hematopoietic stem cell IFL
la2Tg; s896Tg standard conditions Long-pec hematopoietic multipotent progenitor cell IFL
Long-pec hematopoietic stem cell IFL
myb WT standard conditions Prim-25 hematopoietic stem cell ISH
Day 4 caudal hematopoietic tissue ISH
jam3bsa37/sa37 standard conditions Prim-25 Not Detected hematopoietic stem cell ISH
Day 4 caudal hematopoietic tissue ISH
rag1 WT standard conditions Day 4 T cell ISH
jam3bsa37/sa37 standard conditions Day 4 Not Detected T cell ISH
runx1 WT standard conditions Prim-15 hematopoietic stem cell ISH
jam3bsa37/sa37 standard conditions Prim-15 Not Detected hematopoietic stem cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jam3bsa37/sa37 standard conditions 26+ somites dorsal aorta fli1 expression decreased distribution, abnormal
26+ somites macrophage lcp1 expression decreased distribution, abnormal
26+ somites nucleate erythrocyte gata1a expression decreased distribution, abnormal
26+ somites nucleate erythrocyte hbae3 expression decreased distribution, abnormal
26+ somites posterior cardinal vein fli1 expression decreased distribution, abnormal
Prim-5 dorsal aorta efnb2a expression decreased distribution, abnormal
Prim-5 dorsal aorta fli1 expression decreased distribution, abnormal
Prim-5 posterior cardinal vein fli1 expression decreased distribution, abnormal
Prim-5 posterior cardinal vein flt4 expression decreased distribution, abnormal
Prim-15 hematopoietic stem cell runx1 expression absent, abnormal
Prim-25 hematopoietic stem cell myb expression absent, abnormal
Day 4 caudal hematopoietic tissue myb expression decreased amount, abnormal
Day 4 T cell rag1 expression absent, abnormal
jam3bsa37/sa37; la2Tg; s896Tg standard conditions Long-pec hematopoietic multipotent progenitor cell EGFP expression decreased distribution, abnormal
Long-pec hematopoietic multipotent progenitor cell mCherry expression decreased distribution, abnormal
Long-pec hematopoietic stem cell EGFP expression decreased distribution, abnormal
Long-pec hematopoietic stem cell mCherry expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development