Fig. 6

Jam3b regulates the integrin signaling pathway to form HSCs. (A) Fluorescent image of a fli1a:Gal4; UAS:mCherry-itgb1aDN embryo. (B) Expression of runx1 in the DA of mCherry-itgb1aDN (−), or mCherry-itgb1aDN (+) embryos uninjected or injected with ca-faka mRNA. (C) Expression of lrrc15 in the vascular cord of mCherry-itgb1aDN (−) or mCherry-itgb1aDN (+) embryos. (D) Expression of runx1 in the DA of mCherry-itgb1aDN (+) embryos uninjected or injected with lrrc15 mRNA. (E) Time-lapse images of fli1a:GFP in WT or jam3bsa37 embryos. Dotted lines indicate the boundary of eleventh and twelfth somite at each time point. White arrowheads indicate a subset of fli1a:GFP (+) cells that show a rounded morphology. Data are representative of four embryos for each genotype. (F) Expression of lrrc15 in the vascular cord of WT, or jam3bsa37 embryos uninjected or injected with drl mRNA, ca-faka mRNA, or both drl and ca-faka mRNA. (G) Expression of runx1 in the DA of WT, or jam3bsa37 embryos uninjected or injected with ca-faka mRNA or both drl and ca-faka mRNA. (H) Expression of lrrc15 in the vascular cord of WT embryos treated with DMSO or wortmannin. (I) Expression of runx1 in the DA of WT embryos treated with wortmannin, either uninjected or injected with lrrc15 mRNA. Percent distribution of runx1 expression in I is shown in Fig. S8C. Black arrowheads indicate the expression domain of each gene. Numbers in bottom right of panels indicate the number of embryos showing the displayed expression pattern over the total number of analyzed embryos. Scale bars: 50 μm (E); 100 μm (A-D,F-H).