ZFIN ID: ZDB-FIG-191230-1891
Liu et al., 2019 - Combinatorial genetic replenishments in myocardial and outflow tract tissues restore heart function in tnnt2 mutant zebrafish. Biology Open   8(12) Full text @ Biol. Open
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Protruding-mouth

Fig. 3.

Gene expression in OFT is deregulated in tnnt2a mutant zebrafish with myocardial tnnt2a supplementation. (A) Workflow of sample collections for whole-fish RNA-seq of four groups: tnnt2+/−, tnnt2a−/− Tg(myl7:tnnt2a); tnnt2a−/− and Tg(myl7:tnnt2a); tnnt2a+/−. (B) Volcano plot of differentially expressed genes in tnnt2a+/− versus Tg(myl7:tnnt2a); tnnt2a−/−. (C,D) Sarcomere- and OFT-relevant gene expression in dissected heart of tnnt2a+/+, tnnt2a−/−, and Tg(myl7:tnnt2a); tnnt2a−/− after DOX induction at 16 hpf. *P<0.05, ***P<0.001, ****P<0.0001, NS, not significant; n=30 per group. Statistical differences were evaluated using two-way ANOVA. Red asterisks, sarcomere genes in Tg(myl7:tnnt2a); tnnt2a−/− can almost return to wild-type level after DOX induction; red hashes, OFT-relevant genes in Tg(myl7:tnnt2a); tnnt2a−/− are more deviated from wild-type level after DOX induction compared with tnnt2a−/−.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
actc1a fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
bmp2b fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
gata4 fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
gata5 fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
has2 fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
mKate2 fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart IFL
myl7 fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tbx2b fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tbx20 fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tnnc1a fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tnni1b fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tnnt2a fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth Not Detected heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
tpm4a fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart RTPCR
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tnnt2afdu108/fdu108; fdu106Tg standard conditions Protruding-mouth heart actc1a expression increased amount, abnormal
Protruding-mouth heart bmp2b expression decreased amount, abnormal
Protruding-mouth heart gata4 expression increased amount, abnormal
Protruding-mouth heart gata5 expression increased amount, abnormal
Protruding-mouth heart myl7 expression increased amount, abnormal
Protruding-mouth heart tbx2b expression decreased amount, abnormal
Protruding-mouth heart tnnc1a expression decreased amount, abnormal
Protruding-mouth heart tnni1b expression increased amount, abnormal
Protruding-mouth heart tnnt2a expression absent, abnormal
Protruding-mouth heart tpm4a expression increased amount, abnormal
tnnt2afdu108/fdu108; fdu106Tg chemical treatment by environment: doxycycline Protruding-mouth heart actc1a expression amount, ameliorated
Protruding-mouth heart bmp2b expression decreased amount, abnormal
Protruding-mouth heart gata4 expression increased amount, abnormal
Protruding-mouth heart gata5 expression increased amount, abnormal
Protruding-mouth heart has2 expression decreased amount, abnormal
Protruding-mouth heart myl7 expression amount, ameliorated
Protruding-mouth heart tbx20 expression increased amount, abnormal
Protruding-mouth heart tbx2b expression decreased amount, abnormal
Protruding-mouth heart tnnc1a expression decreased amount, abnormal
Protruding-mouth heart tnni1b expression amount, ameliorated
Protruding-mouth heart tnnt2a expression amount, ameliorated
Protruding-mouth heart tpm4a expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Biology Open for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Biol. Open