Suppression of Wnt/β-catenin signaling by Nup62l deprivation activated multiple apoptotic pathways. (A) Apoptotic cells were detected by TUNEL assays at 72 hpf in PA of WT embryos, nup62l-mutants, nup62l-mutants treated with 10 μM Wnt agonist 1, WT treated with 0.15% DMSO or 15 μM XAV939, and WT treated with both 300 ng nup62l mRNA and 15 μM XAV939. A significantly increased apoptosis was observed in nup62l-mutants and WT embryos treated with XAV939. (B) Alcian blue staining at 96 hpf of nup62l-mutants treated with or without 10 μM Wnt agonist 1, WT treated with 0.15% DMSO or 15 μM XAV939, and WT treated with both 300 ng nup62l mRNA and 15 μM XAV939. Note a greatly decreased cartilages in PA of nup62l-mutants and larvae treated with 15 μM XAV939. Images are ventral views with anterior to the left. (C) Relative mRNA levels of genes for apoptotic pathways. Embryos treated as indicated above were collected at 72 hpf for qRT–PCR assays. Data expressed as Mean ± SD were representatives of three independent experiments. ** p < 0.01.
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