ZFIN ID: ZDB-FIG-191003-2
Xue et al., 2019 - A 3D Atlas of Hematopoietic Stem and Progenitor Cell Expansion by Multi-dimensional RNA-Seq Analysis. Cell Reports   27:1567-1578.e5 Full text @ Cell Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Pec-fin to Day 4

Fig. S5

The hematopoietic deficiency in smchd1 morphants, related to figure 3.

A The illustration of smchd1 splice morpholino (sMO) target site which is located between exon 3 and intron 3.

B. The smchd1 sMO validation by RT-PCR. The agarose gel imaging showing the abnormal spliced transcripts in sMO injected embryos compared with control, B-actin used as control.

C. Confocal imaging of the transgenic line (kdrl;mCherry/CD41:GFP) showing the number of CD41 cells in control and smchd1 sMO injected embryos at 4.5 dpf. n=4

D. WISH showing the expression of bel-globin, pu. 1 and cmyb at 2 dpf in control and smchd1 sMO injected embryos.

E. WISH showing the expression of cmyb, gatal and scl at 4dpf in control and smchd1 sMO injected embryos.

F. WISH showing the expression of rag1 and foxn1 at 4.5 dpf in control and smchd1 sMO injected embryos.

G. WISH showing the expression of runx1 at 28 hpf in control and smchd1 ATG morpholino (aMO) injected embryos.

H. WISH showing the expression of cmyb and rag1 in control and smchd1 aMO injected embryos at 4 dpf.

I. The double staining image of anti-BrdU antibody and eGFP FISH in control and smchd1 morphants at 2.5 dpf. White arrowheads indicate draculin+BrdU+ double positive cells. 

J. The statistical data show the percentage of draculin+BrdU+/draculin+ cells in (I). n(control)=4, n(smchd1)=5. Data are shown as means +- SEM. *P<0.05

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP pku6Tg ; la2Tg + MO3-smchd1 control Day 4 caudal hematopoietic tissue IFL
Day 4 caudal hematopoietic tissue hematopoietic multipotent progenitor cell IFL
foxn1 TU + MO1-smchd1 control Day 4 thymic epithelium ISH
gata1a TU + MO1-smchd1 control Day 4 caudal hematopoietic tissue ISH
hbbe1.1 TU + MO1-smchd1 control Long-pec ventral wall of dorsal aorta ISH
myb TU + MO1-smchd1 control Long-pec ventral wall of dorsal aorta ISH
Day 4 caudal hematopoietic tissue ISH
TU + MO3-smchd1 control Day 4 caudal hematopoietic tissue ISH
rag1 TU + MO1-smchd1 control Day 4 thymus ISH
TU + MO3-smchd1 control Day 4 thymus ISH
runx1 TU + MO3-smchd1 control Prim-5 ventral wall of dorsal aorta hematopoietic multipotent progenitor cell ISH
spi1b TU + MO1-smchd1 control Long-pec ventral wall of dorsal aorta ISH
tal1 TU + MO1-smchd1 control Day 4 caudal hematopoietic tissue ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-smchd1 control Day 4 caudal hematopoietic tissue gata1a expression decreased amount, abnormal
Day 4 caudal hematopoietic tissue myb expression decreased amount, abnormal
Day 4 caudal hematopoietic tissue tal1 expression decreased amount, abnormal
Day 4 thymus rag1 expression decreased amount, abnormal
TU + MO3-smchd1 control Day 4 caudal hematopoietic tissue myb expression decreased amount, abnormal
Day 4 thymus rag1 expression decreased amount, abnormal
cz3331Tg + MO1-smchd1 control Pec-fin hematopoietic multipotent progenitor cell cell population proliferation decreased occurrence, abnormal
pku6Tg ; la2Tg + MO3-smchd1 control Day 4 caudal hematopoietic tissue EGFP expression decreased amount, abnormal
Day 4 caudal hematopoietic tissue EGFP expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cell Rep.