ZFIN ID: ZDB-FIG-190823-18
Whitesell et al., 2019 - foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish. Developmental Biology   453(1):34-47 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 4

Fig. 7

Expression of genes with enriched expression in the embryonic vSMC transcriptome. Gene expression from in situhybridization in laterally mounted 4 dpf embryos. Gene of interest mRNA is depicted in green, with αGFP antibody (red) detecting acta2:EGFP transgenic expression in smooth muscle cells. Arrows depict co-expression of gene of interest mRNA with acta2:EGFPexpression. A) tagln is expressed in the aortic arch arteries, and co-expressed with acta2:EGFP. B) capn3b expressed in the aortic arch arteries and brain. C) kcne4 is lowly expressed in the aortic arch arteries. D) pcolce2b is expressed in the aortic arch arteries and the brain. E) cnn1b is expressed in the aortic arch arteries. F) loxa is expressed in the aortic arch arteries, the bulbus arteriosus, and the ventral brain. G) lmod1b is expressed in the aortic arch arteries and brain. H) desmb is expressed in the aortic arch arteries, and in punctate patterns in the brain. I) myl9a is expressed in the aortic arch arteries. J) tpm4b is expressed in the brain and lowly expressed in the aortic arch arteries. K) si:ch211-247i17.1 is expressed in the aortic arch arteries and brain. Scale bar represents 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
capn3b ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
cnn1b ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
desmb ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
EGFP ca7Tg standard conditions Day 4 aortic arch vascular associated smooth muscle cell IHC
Day 4 brain vascular associated smooth muscle cell IHC
kcne4 ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
lmod1b ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
loxa ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain vascular associated smooth muscle cell ISH
Day 4 brain ventral region ISH
Day 4 bulbus arteriosus ISH
myl9a ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
pcolce2b ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
si:ch211-247i17.1 ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
tagln ca7Tg standard conditions Day 4 aortic arch vascular associated smooth muscle cell ISH
tpm4b ca7Tg standard conditions Day 4 aortic arch ISH
Day 4 aortic arch vascular associated smooth muscle cell ISH
Day 4 brain ISH
Day 4 brain vascular associated smooth muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.