FIGURE

Fig. 8

ID

ZDB-FIG-190823-17

Publication

Whitesell et al., 2019 - foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish

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Fig. 8

Necessity of foxc1 for embryonic head vSMC development. A) Ventral images of 3 dpf embryos depicting ventral aorta (red) and smooth muscle coverage (green) in foxc1a/foxc1b mutant genotypes. B) Ventral images of 4 dpf embryos depicting ventral aorta (red) and smooth muscle coverage (green) in foxc1a/foxc1b mutant genotypes. Genotypes are listed in each corresponding panel. C) At 4 dpf, the length of smooth muscle coverage on the ventral aorta is reduced foxc1a^−/− mutants. D) At 4 dpf, the length of the ventral aorta is significantly reduced in foxc1a^−/− mutants. E) Mural cell markers acta2 and pdgfrβ have reduced expression with the loss of foxc1a, and a stronger effect with the combined loss of foxc1a and foxc1b, compared to wildtype and loss of foxc1b alone at 4dpf. F) Compared to wildtype embryos, expression of foxc1a and foxc1b are both decreased in foxc1a mutants. The combined loss of both foxc1a and foxc1b results in a lack of expression of foxc1a or foxc1b. G) myl9aexpression in the aortic arch arteries is reduced with the loss of foxc1a, either in foxc1a mutants, or in combined foxc1a/foxc1bmutants. *** = p < 0.0001, ANOVA with Dunnett's multiple comparisons test. Scale bar represents 50 μm.

Expression Data

Genes:	acta2 foxc1a foxc1b myl9a pdgfrb
Fish:	WT foxc1a^{ua1017/ua1017} foxc1b^{ua1018/ua1018} foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}
Anatomical Terms:	aortic arch pericyte vascular associated smooth muscle cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxc1a^{ua1017/ua1017} foxc1a^ua1017/+; ca7Tg/+; ci5Tg/+ foxc1a^{ua1017/ua1017}; ca7Tg/+; ci5Tg/+ foxc1b^ua1018/+; ca7Tg/+; ci5Tg/+ foxc1b^{ua1018/ua1018}; ca7Tg/+; ci5Tg/+ foxc1a^ua1017/+; foxc1b^ua1018/+; ca7Tg/+; ci5Tg/+ foxc1a^ua1017/+; foxc1b^{ua1018/ua1018}; ca7Tg/+; ci5Tg/+ foxc1a^{ua1017/ua1017}; foxc1b^ua1018/+; ca7Tg/+; ci5Tg/+ foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}; ca7Tg/+; ci5Tg/+
Observed In:	aortic arch pericyte vascular associated smooth muscle cell ventral aorta ventral aorta vascular associated smooth muscle cell
Stage Range:	Protruding-mouth to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.