FIGURE

Fig. 2

ID

ZDB-FIG-190823-21

Publication

Whitesell et al., 2019 - foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Timelapse of foxc1b expressing mesenchymal cells attaching to the endothelium followed by upregulation of acta2. A) Ventral views of aortic arch arteries of a foxc1b:EGFP; kdrl:mCherry embryo using confocal time-lapse imaging starting at 50 hpf. Mesenchymal cells are indicated by the asterisk, while vascular mural cells are indicated by arrowheads, starting by 56–58 hpf. B) Orthogonal views at 58 hpf depict the foxc1b:EGFP cells wrapping around, but not co-expressing an endothelial marker. C) Time-lapse of the early ventral aorta of a foxc1b:EGFP; acta2:mCherry embryo from 60 to 70 hpf. foxc1b:EGFP positive smooth muscle cells are associated with the endothelium (arrowheads) but do not co-express acta2:mCherry until approximately 66 hpf (arrow). D) Single channel images of acta2:mCherry. E) Orthogonal views at 70 hpf show acta2:mCherry co-expressed within a foxc1b:EGFP positive cell. Scale bars represent 20 μm.

Expression Data

Genes:	EGFP mCherry
Fish:	ca8Tg; mw44Tg ci5Tg; mw44Tg
Anatomical Terms:	aortic arch blood vessel endothelium aortic arch vascular associated smooth muscle cell ventral aorta blood vessel endothelium ventral aorta vascular associated smooth muscle cell
Stage Range:	Long-pec to Pec-fin

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.