Fig. S9

Expression of new and known smooth muscle markers. Gene expression using colorimetric ISH in lateral view wholemount and sections, compared to endothelial (antibody staining for kdrl:EGFP, brown, column i), or smooth muscle (acta2:EGFP, brown, column ii) transgenes. Arrowheads depict gene expression associated with the endothelium, arrows depict gene expression co-expressed with smooth muscle. A) tagln, a known smooth muscle marker, is found in the aortic arch arteries, notochord tip, swim bladder, and gut in wholemount. In sections tagln is expressed in cells adjacent to the endothelium and is co-expressed with acta2:EGFP on the ventral aorta and aortic arch arteries. B) capn3b is expressed in cells adjacent to the endothelium in the brain. C) kcne4 is expressed adjacent to the endothelium and is co-expressed in smooth muscle on the ventral aorta and aortic arch arteries. D) pcolce2b is expressed adjacent to the endothelium and is co-expressed with acta2:EGFP. E) cnn1b is expressed adjacent to the endothelium of the aortic arch arteries. F) loxa is expressed solely in the smooth muscle of the bulbus arteriosus. G) lmod1b is expressed adjacent to the endothelium and overlaps with acta2:EGFP. H) desmb is expressed in a punctate pattern in the brain. I) myl9a is expressed adjacent to the endothelium, and partially overlaps with acta2:EGFP. J) tpm4b is expressed near the endothelium in the ventral head and is co-expressed with acta2:EGFP in the brain. K) si:ch211-247i17.1 is expressed near the endothelium and is co-expressed with acta2:EGFP. Scale bar for A-K (wholemount) represent 200 µm. Scale bars for sections (i and ii) represent 20 µm.