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Fig. 7

Expression of genes with enriched expression in the embryonic vSMC transcriptome. Gene expression from in situhybridization in laterally mounted 4 dpf embryos. Gene of interest mRNA is depicted in green, with αGFP antibody (red) detecting acta2:EGFP transgenic expression in smooth muscle cells. Arrows depict co-expression of gene of interest mRNA with acta2:EGFPexpression. A) tagln is expressed in the aortic arch arteries, and co-expressed with acta2:EGFP. B) capn3b expressed in the aortic arch arteries and brain. C) kcne4 is lowly expressed in the aortic arch arteries. D) pcolce2b is expressed in the aortic arch arteries and the brain. E) cnn1b is expressed in the aortic arch arteries. F) loxa is expressed in the aortic arch arteries, the bulbus arteriosus, and the ventral brain. G) lmod1b is expressed in the aortic arch arteries and brain. H) desmb is expressed in the aortic arch arteries, and in punctate patterns in the brain. I) myl9a is expressed in the aortic arch arteries. J) tpm4b is expressed in the brain and lowly expressed in the aortic arch arteries. K) si:ch211-247i17.1 is expressed in the aortic arch arteries and brain. Scale bar represents 50 μm.

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Reprinted from Developmental Biology, 453(1), Whitesell, T.R., Chrystal, P.W., Ryu, J.R., Munsie, N., Grosse, A., French, C.R., Workentine, M.L., Li, R., Zhu, L.J., Waskiewicz, A., Lehmann, O.J., Lawson, N.D., Childs, S.J., foxc1 is required for embryonic head vascular smooth muscle differentiation in zebrafish, 34-47, Copyright (2019) with permission from Elsevier. Full text @ Dev. Biol.