FIGURE

Fig. 3

ID

ZDB-FIG-190723-1350

Publication

Pociute et al., 2019 - Clec14a genetically interacts with Etv2 and Vegf signaling during vasculogenesis and angiogenesis in zebrafish

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Fig. 3

Analysis of vascular marker expression by in situ hybridization at 24 hpf in clec14a−/− embryos injected with 10 ng of c1qr MO. a-d kdrl, e-h fli1a, i-l cdh5, m-p arterial marker cldn5b, q-t venous marker flt4expression. Note the strong inhibition of angiogenic sprouting in the double clec14a−/−; c1qr MO embryos (arrowheads). Only weak or partial inhibition of sprouting is observed in clec14a−/− or c1qrMO embryos. u Percentage of embryos with defects in ISV sprouting is greatly increased among c1qrMO; clec14a−/− embryos compared to c1qr MO or clec14a−/− embryos. n refers to the number of embryos analyzed for each marker. *p < 0.05; ** p < 0.01; *** p < 0.001; NS, no significance, Fisher’s exact test. Data were combined from two independent experiments. Error bars show standard error

Expression Data

Genes:	cdh5 cldn5b fli1 flt4 kdrl
Fish:	WT clec14a^ci15/ci15 WT + MO1-cd93 clec14a^ci15/ci15 + MO1-cd93
Knockdown Reagent:	MO1-cd93
Anatomical Terms:	artery axial vasculature dorsal aorta intersegmental vessel posterior cardinal vein vein
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	clec14a^ci15/ci15 + MO1-cd93
Knockdown Reagent:	MO1-cd93
Observed In:	intersegmental vessel
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.