Fig. 5

clec14a mutation and vegfaa MO knockdown show synergistic effect in inhibiting intersegmental vessel (ISV) sprouting. a-d ISH analysis of kdrl expression at 24 hpf. e-h Analysis of kdrl: GFP expression at 48 hpf. Note that partial inhibition of sprouting is observed using low dose of 2.5 ng vegfaa MO in wt embryos (c, g). The same dose in clec14a mutants results in a complete loss of sprouting at 24 hpf and significant inhibition of sprouting at 48 hpf (d, h). i Percentage of embryos with normal or inhibited ISVs based on kdrl expression at 24 hpf. *** p < 0.001, Fisher’s exact test; embryos with partially reduced ISVs were compared between wt and clec14a−/− or vegfaa MO groups; embryos with no ISVs were compared between the double knockdown vegfaa MO; clec14a−/− and the other groups. Data were combined from two independent experiments