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ZFIN ID: ZDB-FIG-190328-13
Boyle Anderson et al., 2018 - A transcriptomics analysis of the Tbx5 paralogues in zebrafish. PLoS One   13:e0208766 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to 20-25 somites

Fig. S4

Complete somite differential expression.

In situ hybridization of all genes differentially expressed in the somites. (a-a”’) At 18hpf, phlda2 is upregulated in Tbx5a-deficient (a’) and Tbx5b-deficient (a”) embryos compared to wildtype embryos (a’), but downregulated in the double-deficient embryos (a”’). At 18hpf, mybphb shows upregulation in Tbx5a-deficent (b’) and double-deficient (b”’) embryos compared to wildtype embryos (b). At 18hpf, hsp90aa1.2 is upregulated in Tbx5a-deficient (c’), Tbx5b-deficient (c”) and double-deficient (c”’) embryos compared to wildtype (c) embryos, especially in the anterior somites. At 21 hpf, pvalb2 expression is increased in Tbx5b-deficient (d”) and double-deficient (d”’) embryos compared to wildtype (d) but not Tbx5a-deficient embryos (d’). At 21 hpf, si:dkey-4p:15.3 expression is increased in Tbx5a-deficient (e’), Tbx5b-deficient (e”) and double-deficient (e”’) embryos. At 21 hpf, obsl1a expression is upregulated in Tbx5a-deficient (f’), Tbx5b-deficient (f”) and double-deficient (f”’) embryos compared to wildtype (f) embryos. At 21 hpf, ctsc expression is expanded in Tbx5a-deficient (g’) and Tbx5b-deficient (g”) embryos compared to wildtype (g) embryos. At 21 hpf, aimp1 expression is decreased in Tbx5a-deficient (h’), Tbx5b-deficient (h”) and double-deficient (h”’) embryos compared to wildtype embryos (h). At 21 hpf, ryr1b expression is increased in both Tbx5a-deficient (i’) and double-deficient (i”’) embryos compared to wildtype (i) embryos. (j-k) Comparison of length between Tbx5b mutant embryos and siblings, n = 10, measurements in μm. (j) Tbx5b-deficient embryos at 3 dpf are significantly shorter than their siblings. (k) Somite size is not significantly different at 3dpf between Tbx5b-deficient embryos and their wildtype siblings. Since somite size varies along the AP axis, measurements were taken of the more anterior somites only. (l) At 25 hpf, there is a significant difference in somite number between wildtype and Tbx5b morpholino injected embryos. Scale bar is 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aimp1a AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
ctsc AB standard conditions 20-25 somites somite medial side ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite medial side ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite medial side ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite medial side ISH
hsp90aa1.2 AB standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite ISH
AB + MO2-tbx5a standard conditions 14-19 somites somite ISH
hspa8b AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
mybphb AB standard conditions 14-19 somites somite dorsal side ISH
AB + MO1-tbx5b standard conditions 14-19 somites somite ISH
14-19 somites somite dorsal side ISH
14-19 somites somite ventral side ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite ISH
14-19 somites trunk anterior region ISH
14-19 somites whole organism posterior region ISH
AB + MO2-tbx5a standard conditions 14-19 somites head ISH
14-19 somites somite ISH
obsl1a AB standard conditions 20-25 somites immature eye ISH
20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites trunk somite ISH
20-25 somites whole organism posterior region ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites Not Detected immature eye ISH
20-25 somites somite ISH
phlda2 AB standard conditions 14-19 somites head mesenchyme ISH
14-19 somites somite ISH
14-19 somites whole organism anterior side ISH
AB + MO1-tbx5b standard conditions 14-19 somites somite ISH
14-19 somites whole organism anterior side ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite ISH
14-19 somites whole organism ISH
AB + MO2-tbx5a standard conditions 14-19 somites somite ISH
14-19 somites whole organism anterior side ISH
pvalb2 AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
ryr1b AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-tbx5b standard conditions 14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
14-19 somites somite mybphb expression increased distribution, abnormal
14-19 somites somite hsp90aa1.2 expression mislocalised, abnormal
14-19 somites somite ventral side mybphb expression mislocalised, abnormal
14-19 somites whole organism anterior side phlda2 expression decreased amount, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites somite hspa8b expression increased amount, abnormal
20-25 somites somite pvalb2 expression increased amount, abnormal
20-25 somites somite hspa8b expression increased distribution, abnormal
20-25 somites somite medial side ctsc expression increased distribution, abnormal
20-25 somites trunk somite obsl1a expression decreased amount, abnormal
20-25 somites whole organism posterior region obsl1a expression increased amount, abnormal
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite phlda2 expression decreased amount, abnormal
14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
14-19 somites somite hsp90aa1.2 expression mislocalised, abnormal
14-19 somites somite mybphb expression spatial pattern, abnormal
14-19 somites trunk anterior region mybphb expression increased amount, abnormal
14-19 somites whole organism phlda2 expression decreased amount, abnormal
14-19 somites whole organism posterior region mybphb expression decreased amount, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites somite ryr1b expression increased amount, abnormal
20-25 somites somite obsl1a expression increased amount, abnormal
20-25 somites somite pvalb2 expression increased amount, abnormal
20-25 somites somite hspa8b expression increased amount, abnormal
20-25 somites somite hspa8b expression increased distribution, abnormal
20-25 somites somite medial side ctsc expression increased distribution, abnormal
AB + MO2-tbx5a standard conditions 14-19 somites head mybphb expression increased amount, abnormal
14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
14-19 somites somite mybphb expression increased amount, abnormal
14-19 somites whole organism anterior side phlda2 expression decreased amount, abnormal
20-25 somites immature eye obsl1a expression absent, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites somite hspa8b expression increased amount, abnormal
20-25 somites somite obsl1a expression increased amount, abnormal
20-25 somites somite hspa8b expression increased distribution, abnormal
20-25 somites somite medial side ctsc expression increased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One