ZFIN ID: ZDB-PUB-181212-15
A transcriptomics analysis of the Tbx5 paralogues in zebrafish
Boyle Anderson, E.A.T., Ho, R.K.
Date: 2018
Source: PLoS One   13: e0208766 (Journal)
Registered Authors: Ho, Robert K.
Keywords: none
Microarrays: GEO:GSE115640
MeSH Terms:
  • Animals
  • Computational Biology
  • Eye/growth & development
  • Eye/metabolism
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Knockdown Techniques
  • Heart/growth & development
  • Myocardium/metabolism
  • T-Box Domain Proteins/genetics
  • T-Box Domain Proteins/metabolism*
  • Transcriptome
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 30532148 Full text @ PLoS One
TBX5 is essential for limb and heart development. Mutations in TBX5 are associated with Holt-Oram syndrome in humans. Due to the teleost specific genome duplication, zebrafish have two copies of TBX5: tbx5a and tbx5b. Both of these genes are expressed in regions of the lateral plate mesoderm and retina. In this study, we perform comparative RNA sequencing analysis on zebrafish embryos during the stages of lateral plate mesoderm migration. This work shows that knockdown of the Tbx5 paralogues results in altered gene expression in many tissues outside of the lateral plate mesoderm, especially in the somitic mesoderm and the intermediate mesoderm. Specifically, knockdown of tbx5b results in changes in somite size, in the differentiation of vasculature progenitors and in later patterning of trunk blood vessels.