Fig. 3

Effects of Tbx5 paralogues on vasculature.

(a-a??) Dorsal view of sox7 expression at 18 hpf showing misexpression in Tbx5a-deficient embryos (a?), Tbx5b-deficient embryos (a?) and double-deficient embryos (a??) compared to normal wildtype expression (a). (b-b??) Dorsal view of hhex expression at 21 hpf displaying mild misexpression in Tbx5a-deficient embryos (b?) and more severe misexpression in Tbx5b-deficient embryos (b?) and double-deficient embryos (b??) compared to wildtype (b). (c-c??) Lateral view of cox6b1 expression at 18 hpf in the developing pronephric duct. Arrowheads mark the anterior limit of expression. Expanded expression is present in the surrounding tissue of Tbx5a-deficient (c?) and double-deficient (c??) embryos, while expression is decreased throughout the embryo in Tbx5b-deficient embryos (c?). (d-d??) Lateral view of the Tg(fli1a::EGFP)y1 line displays increased branching in the intersomitic vessels of both Tbx5b-deficient (d?) and double-deficient embryos (d??) (see arrowheads) compared to either wildtype (d) or Tbx5a-deficient embryos (d?). (e-e??) Dorsal view of the Tg(fli1a::EGFP)y1 line. The subintestinal vessels are marked with a bracket in 3 dpf embryos. There is a decrease in size of the subintestinal vasculature in Tbx5b-deficient (e?) and double-deficient (e??) embryos compared to either wildtype (e) or Tbx5a-deficient embryos (e?). Scale bars are 100 ?m.