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ZFIN ID: ZDB-FIG-190328-11
Boyle Anderson et al., 2018 - A transcriptomics analysis of the Tbx5 paralogues in zebrafish. PLoS One   13:e0208766 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 4

Effects of Tbx5 Paralogues on the Somites.

All views are lateral, see text for details. hsp90aa1.2 expression at 18 somite stage is expanded in Tbx5b-deficient (a”) and double-deficient (a”’) embryos compared to wildtype (a). phlda2 expression at 18 hpf is consistent in the somites in wildtype (b), Tbx5a-deficient (b’) and Tbx5b-deficient (b”) embryos, but mostly absent in double-deficient embryos(b”’). Expression of aimp1 at the 21 hpf stage is decreased in Tbx5a-deficient (c’), Tbx5b-deficient (c”) and double-deficient (c”’) embryos compared to wildtype embryos (c). In 21 hpf embryos, obsl1a expression is increased in the somites of Tbx5a-deficient (d’) and double-deficient (d”’) embryos compared to wildtype (d) embryos. Tbx5b-deficient embryos show a decrease in expression in the trunk somites (d”) but an increase in the more posterior somites (S4F” Fig) compared to wildtype embryos. Expression of ryr1b is increased slightly in the Tbx5a-deficient (e’) embryos compared to wildtype embryos (e) and most strongly in the double-deficient embryos especially in the ventral somites(e”’). Expression of ryr1b in the Tbx5b-deficient embryos (e”’) is more similar to wildtype than to Tbx5a-deficient embryos. Scale bar is 100 μm. (f) Somite count of embryos at 25 hpf. Tbx5a-deficient (n = 16), Tbx5b-deficient (n = 12), and double-deficient (n = 15) showed no significant difference in somite count compared to wildtype (n = 15). (g) Somite length measured along the AP axis of wt (n = 104), Tbx5a-deficient (n = 110), Tbx5b-deficient (n = 66) and double-deficient (n = 96). Both Tbx5b-deficient and double-deficient embryos showed a significant decrease in somite size compared to wt embryos. (g-f) significance was tested using ANOVA and Tukey HSD test, *p<0.05, ***p<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aimp1a AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
hsp90aa1.2 AB standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite ISH
AB + MO2-tbx5a standard conditions 14-19 somites somite ISH
obsl1a AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites trunk somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
phlda2 AB standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b standard conditions 14-19 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite ISH
AB + MO2-tbx5a standard conditions 14-19 somites somite ISH
ryr1b AB standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b standard conditions 20-25 somites somite ISH
AB + MO1-tbx5b + MO2-tbx5a standard conditions 20-25 somites somite ISH
AB + MO2-tbx5a standard conditions 20-25 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-tbx5b standard conditions 14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
14-19 somites somite hsp90aa1.2 expression mislocalised, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites trunk somite obsl1a expression decreased amount, abnormal
Prim-5 somite amount, normal
Prim-5 somite decreased size, abnormal
AB + MO1-tbx5b + MO2-tbx5a standard conditions 14-19 somites somite phlda2 expression decreased amount, abnormal
14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
14-19 somites somite hsp90aa1.2 expression mislocalised, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites somite ryr1b expression increased amount, abnormal
20-25 somites somite obsl1a expression increased amount, abnormal
Prim-5 somite amount, normal
Prim-5 somite decreased size, abnormal
AB + MO2-tbx5a standard conditions 14-19 somites somite hsp90aa1.2 expression increased amount, abnormal
20-25 somites somite aimp1a expression decreased amount, abnormal
20-25 somites somite obsl1a expression increased amount, abnormal
Prim-5 somite amount, normal
Prim-5 somite size, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One