ZFIN ID: ZDB-FIG-180912-57
Neelathi et al., 2018 - Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.. Development (Cambridge, England)   145(14) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: 26+ somites
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: 26+ somites

Fig. 6

Delayed Wnt Fgf signaling dynamics in stalled primordia. (A) In control embryos, the primordium (pLLp) migrates and separates from the pLL ganglion (pLLg) by 22 hpf and lef1 expression is restricted to the leading domain. (B,C) With migration inhibited in cxcl12a morphants and PP1-treated embryos, the primordium remains juxtaposed with the pLLg and lef1 expression remains broad. (D) pea3 expression at the trailing end of the primordium in controls at 22 hpf. (E,F) The pea3 expression domain is still at the trailing end of the prospective primordium in the absence of migration; however, the pea3 domain is in the middle of a broad lef1-expressing domain, which now represents the juxtaposed primordium and the pLLg. (G-I) snail1b expression in control, cxcl12a morphant and PP1-treated primordia at 22 hpf. OV, otic vesicle. n=25 for each of two independent experiments. Dotted lines encircle pLL primordia.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
etv4 zf106Tg control 26+ somites posterior lateral line primordium anterior region ISH
zf106Tg chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 26+ somites posterior lateral line primordium central region ISH
zf106Tg + MO4-cxcl12a standard conditions 26+ somites posterior lateral line primordium central region ISH
lef1 zf106Tg control 26+ somites posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 26+ somites posterior lateral line primordium ISH
zf106Tg + MO4-cxcl12a standard conditions 26+ somites posterior lateral line primordium ISH
snai1b zf106Tg control 26+ somites posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 26+ somites posterior lateral line primordium posterior region ISH
zf106Tg + MO4-cxcl12a standard conditions 26+ somites posterior lateral line primordium posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg chemical treatment by environment: 1-tert-butyl-3-(4-methylphenyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine 26+ somites posterior lateral line ganglion fused with posterior lateral line placode, abnormal
26+ somites posterior lateral line primordium lef1 expression increased amount, abnormal
26+ somites posterior lateral line primordium lef1 expression increased distribution, abnormal
26+ somites posterior lateral line primordium central region etv4 expression increased amount, abnormal
26+ somites posterior lateral line primordium central region etv4 expression mislocalised, abnormal
26+ somites posterior lateral line primordium posterior lateral line neuromast primordium migration decreased rate, abnormal
zf106Tg + MO4-cxcl12a standard conditions 26+ somites posterior lateral line ganglion fused with posterior lateral line placode, abnormal
26+ somites posterior lateral line primordium lef1 expression increased amount, abnormal
26+ somites posterior lateral line primordium lef1 expression increased distribution, abnormal
26+ somites posterior lateral line primordium central region etv4 expression increased amount, abnormal
26+ somites posterior lateral line primordium central region etv4 expression mislocalised, abnormal
26+ somites posterior lateral line primordium posterior lateral line neuromast primordium migration arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development