ZFIN ID: ZDB-FIG-180912-52
Neelathi et al., 2018 - Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.. Development (Cambridge, England)   145(14) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Prim-15

Fig. 1

Wnt-dependent Fgf signaling inhibits snail1b expression in the trailing domain. (A,B) Expression of snail1b and Wnt-dependent lef1 in leading domain of pLL primordium in control DMSO-treated embryos at 32 hpf. Arrow and arrowhead show snail1b in the pLL primordium and in the underlying horizontal myoseptum cells, respectively. (C,D) snail1b and lef1 expression in BIO-treated embryos. (E-J) snail1b and lef1 expression following heat shock in HS:dkk1b embryos (E,F), without heat shock in HS:dkk1b embryos (G,H) and with heat shock in non-transgenic siblings (I,J). (K,L) Expression of snail1b and Fgf-dependent pea3 at 32 hpf in control DMSO-treated embryos. (M,N) snail1b and pea3 expression in SU5402-treated embryos. (O-T) snail1b and pea3 expression following heat shock in HS:DN-fgfr1 embryos (O,P), without heat shock in HS:DN-fgfr1 embryos (Q,R), and with heat shock in non-transgenic siblings (S,T). Fluorescent anti-GFP staining in the Tg[cldnb:lynGFP] line was used to define the outline of all pLL primordia (dotted line). The primordia leading end is always to the right. n=20 for each of three independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
etv4 zf106Tg control Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 Not Detected posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: SU5402 Prim-15 Not Detected posterior lateral line primordium anterior region ISH
Prim-15 Not Detected posterior lateral line primordium central region ISH
zf106Tg heat shock Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 Not Detected posterior lateral line primordium posterior region ISH
zf106Tg ; pd1Tg standard conditions Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 Not Detected posterior lateral line primordium posterior region ISH
zf106Tg ; pd1Tg heat shock Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
lef1 w32Tg; zf106Tg standard conditions Prim-15 posterior lateral line primordium posterior region ISH
w32Tg; zf106Tg heat shock Prim-15 Not Detected posterior lateral line primordium ISH
zf106Tg control Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: 6-bromoindirubin-3'-oxime Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg heat shock Prim-15 posterior lateral line primordium posterior region ISH
snai1b w32Tg; zf106Tg standard conditions Prim-15 posterior lateral line primordium posterior region ISH
w32Tg; zf106Tg heat shock Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg control Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: 6-bromoindirubin-3'-oxime Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg heat shock Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg ; pd1Tg standard conditions Prim-15 posterior lateral line primordium posterior region ISH
zf106Tg ; pd1Tg heat shock Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w32Tg; zf106Tg heat shock Prim-15 posterior lateral line primordium lef1 expression absent, abnormal
Prim-15 posterior lateral line primordium anterior region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium posterior lateral line development decreased process quality, abnormal
zf106Tg chemical treatment by environment: 6-bromoindirubin-3'-oxime Prim-15 posterior lateral line primordium anterior region lef1 expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region lef1 expression mislocalised, abnormal
Prim-15 posterior lateral line primordium posterior lateral line development decreased process quality, abnormal
zf106Tg chemical treatment by environment: SU5402 Prim-15 posterior lateral line primordium anterior region etv4 expression absent, abnormal
Prim-15 posterior lateral line primordium anterior region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region etv4 expression absent, abnormal
Prim-15 posterior lateral line primordium central region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium posterior lateral line development decreased process quality, abnormal
zf106Tg ; pd1Tg heat shock Prim-15 posterior lateral line primordium anterior region etv4 expression decreased amount, abnormal
Prim-15 posterior lateral line primordium anterior region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region etv4 expression decreased amount, abnormal
Prim-15 posterior lateral line primordium central region snai1b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium posterior lateral line development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development