ZFIN ID: ZDB-FIG-180912-56
Neelathi et al., 2018 - Cxcl12a induces snail1b expression to initiate collective migration and sequential Fgf-dependent neuromast formation in the zebrafish posterior lateral line primordium.. Development (Cambridge, England)   145(14) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

Delayed Wnt Fgf signaling dynamics in the primordium of snail1b morphants. (A-C) Progressive restriction and eventual loss of lef1 expression in controls from 26 to 48 hpf. (D-F) Delayed restriction and persistent lef1 expression in snail1b morphants. (G-I) pea3 expression from 26 to 48 hpf in control embryos. (J-L) Delayed establishment and persistent trailing expression of pea3 expression in snail1b morphants. (M-O) cxcr4b expression from 26 to 48 hpf in control embryos. (P-R) Broad persistent cxcr4b expression in snail1b morphants. (S-U) cxcr7b expression from 26 to 48 hpf in control embryos. (V-X) Delayed establishment and persistent cxcr7b expression in snail1b morphants. (Y) Graph of length of the lef1 expression domain in control and snail1b-MO embryos plotted against the distance migrated by the pLL primordium. n=25 for each of two independent experiments if not stated in the text, n=147 and 144 for control and snail1b-MO for the data shown in Y. Dotted lines encircle pLL primordia.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
ackr3b zf106Tg control Prim-5 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium anterior region ISH
Long-pec Not Detected posterior lateral line primordium ISH
zf106Tg + MO1-snai1b standard conditions Prim-5 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium anterior region ISH
Long-pec posterior lateral line primordium anterior region ISH
cxcr4b zf106Tg control Prim-5 posterior lateral line primordium posterior region ISH
Prim-15 posterior lateral line primordium posterior region ISH
Long-pec posterior lateral line primordium posterior region ISH
zf106Tg + MO1-snai1b standard conditions Prim-5 posterior lateral line primordium anterior region ISH
Prim-5 posterior lateral line primordium central region ISH
Prim-5 posterior lateral line primordium posterior region ISH
Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
Long-pec posterior lateral line primordium anterior region ISH
Long-pec posterior lateral line primordium central region ISH
Long-pec posterior lateral line primordium posterior region ISH
etv4 zf106Tg control Prim-5 posterior lateral line primordium anterior region ISH
Prim-5 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Long-pec posterior lateral line primordium anterior region ISH
Long-pec posterior lateral line primordium central region ISH
Long-pec posterior lateral line primordium posterior region ISH
zf106Tg + MO1-snai1b standard conditions Prim-5 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium anterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Long-pec posterior lateral line primordium anterior region ISH
Long-pec posterior lateral line primordium central region ISH
Long-pec posterior lateral line primordium posterior region ISH
lef1 zf106Tg control Prim-5 posterior lateral line primordium posterior region ISH
Prim-15 posterior lateral line primordium posterior region ISH
Long-pec Not Detected posterior lateral line primordium ISH
zf106Tg + MO1-snai1b standard conditions Prim-5 posterior lateral line primordium anterior region ISH
Prim-5 posterior lateral line primordium central region ISH
Prim-5 posterior lateral line primordium posterior region ISH
Prim-15 posterior lateral line primordium central region ISH
Prim-15 posterior lateral line primordium posterior region ISH
Long-pec posterior lateral line primordium posterior region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
zf106Tg + MO1-snai1b standard conditions Prim-5 posterior lateral line primordium anterior region ackr3b expression decreased amount, abnormal
Prim-5 posterior lateral line primordium anterior region ackr3b expression decreased distribution, abnormal
Prim-5 posterior lateral line primordium anterior region cxcr4b expression increased amount, abnormal
Prim-5 posterior lateral line primordium anterior region cxcr4b expression mislocalised, abnormal
Prim-5 posterior lateral line primordium anterior region etv4 expression decreased amount, abnormal
Prim-5 posterior lateral line primordium anterior region lef1 expression increased amount, abnormal
Prim-5 posterior lateral line primordium anterior region lef1 expression mislocalised, abnormal
Prim-5 posterior lateral line primordium central region cxcr4b expression increased amount, abnormal
Prim-5 posterior lateral line primordium central region cxcr4b expression mislocalised, abnormal
Prim-5 posterior lateral line primordium central region lef1 expression increased amount, abnormal
Prim-5 posterior lateral line primordium central region lef1 expression mislocalised, abnormal
Prim-5 posterior lateral line primordium posterior region lef1 expression increased amount, abnormal
Prim-5 posterior lateral line primordium posterior region lef1 expression increased distribution, abnormal
Prim-15 posterior lateral line primordium anterior region cxcr4b expression increased amount, abnormal
Prim-15 posterior lateral line primordium anterior region cxcr4b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region cxcr4b expression increased amount, abnormal
Prim-15 posterior lateral line primordium central region cxcr4b expression mislocalised, abnormal
Prim-15 posterior lateral line primordium central region lef1 expression increased amount, abnormal
Prim-15 posterior lateral line primordium central region lef1 expression mislocalised, abnormal
Prim-15 posterior lateral line primordium posterior region lef1 expression increased amount, abnormal
Prim-15 posterior lateral line primordium posterior region lef1 expression increased distribution, abnormal
Long-pec posterior lateral line primordium anterior region ackr3b expression increased amount, abnormal
Long-pec posterior lateral line primordium anterior region ackr3b expression mislocalised, abnormal
Long-pec posterior lateral line primordium anterior region cxcr4b expression increased amount, abnormal
Long-pec posterior lateral line primordium anterior region cxcr4b expression mislocalised, abnormal
Long-pec posterior lateral line primordium central region cxcr4b expression increased amount, abnormal
Long-pec posterior lateral line primordium central region cxcr4b expression mislocalised, abnormal
Long-pec posterior lateral line primordium central region etv4 expression decreased amount, abnormal
Long-pec posterior lateral line primordium posterior region etv4 expression decreased amount, abnormal
Long-pec posterior lateral line primordium posterior region lef1 expression increased amount, abnormal
Long-pec posterior lateral line primordium posterior region lef1 expression mislocalised, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development