FIGURE

Fig. 4

ID

ZDB-FIG-180724-25

Publication

Schredelseker et al., 2018 - Bsx controls pineal complex development

Other Figures

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Fig. 4

Absence of parapineal cell cluster and habenula right-isomerism in embryos homozygous for a targeted bsx mutant allele. (A-G) Expression of PC or habenula markers analyzed by WISH in wild-type and bsx mutant embryos at 3?dpf (A-B?,D-E?,G,G?), 34?hpf (C,C?) or 5?dpf (F,F?). Arrowheads indicate the location of PP cells. All embryos were genotyped [n?4 each for wild type (wt) and mutants]. Dorsal views, with the anterior at the top. (H) Time series of pineal anlage in Tg(foxd3:GFP) at stages (indicated at left) when the PP develops. In wt but not bsx mutant embryos, GFP⁺ cells migrate out of the anterior pineal anlage to form a PP cell cluster (arrowhead). Widefield epifluorescence images (20� lens, NA 1.0) of PC, dorsal views, with the anterior at the top. L, left; R, right. Scale bars: 100?�m (A-G) and 50?�m (H).

Expression Data

Genes:	adcyap1a gfi1ab GFP kctd12.1 kctd12.2 lhx2b otx5 slc18a3b
Fish:	AB/TL bsx^m1376/m1376 (AB/TL) zf15Tg (AB/TL) bsx^m1376/m1376; zf15Tg (AB/TL)
Anatomical Terms:	epiphysis habenula left side habenula right side parapineal organ pineal complex
Stage Range:	Prim-15 to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	bsx^m1376/m1376 (AB/TL) bsx^m1376/m1376; zf15Tg (AB/TL)
Observed In:	epiphysis habenula determination of left/right asymmetry in diencephalon habenula left side habenula development parapineal organ parapineal organ animal organ formation pineal complex pineal complex animal organ formation
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

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