Fig. S4
bsx expression in embryos with altered Nodal signaling. (A-D) bsx expression analyzed by whole mount in situ hybridization, dorsal views, anterior at top; scale bar: 50 µm. (A) bsx is expressed normally (see Fig. 1D) in the pineal gland in GFP encoding mRNA injected wildtype control embryos at 30 hpf (n=18/18). (B) In embryos injected with lefty mRNA, bsx is expressed in two bilateral domains in the putative pineal anlage at 30 hpf in embryos (n=23/26), which may be caused by the fact that the neural tube fails to close in lefty overexpressing embryos. (C) At 2 dpf bsx is expressed asymmetrically in the parapineal on the left side of the pineal complex of wildtype siblings or ntlb160 heterozygous embryos (phenotypically considered wildtype siblings). (D) in contrast, in ntlb160 homozygous siblings, sidedness of parapineal bsx expression appears to be randomized, as the bsx domain was found in n=5/12 embryos on the right side of the pineal (parapineal cells were displaced to the left in 6/12 embryos; 1/12 embryos showed bsx expression only in the pineal gland and not displaced from the midline). |