Fig. S4

Blood flow tuning does not affect the architecture of the caudal plexus or tumor cells ability to migrate and adhere. (A) 3D segmentation (IMOD) from one zebrafish posterior caudal plexus per condition (See also Movie 12). (B) Total vascular surface from dorsal aorta (DA, red on A) and caudal veins (CV, blue on A) for each condition (n=3). (C) Number of vascular loops for each condition (n=3). (D) Representative images of embryos injected with dextran 500kDa and imaged 3 hpi are shown (E) Representative images of wound closure for each condition 0, 9 and 24 hours after wound. (F) Wound closure over time (n=3). (G) Representative image of TC stained with crystal violet during the adhesion test (left). Absorbance measured at 590nm (right). (H) Number of adhesion events per field after 10 min of perfusion of tumor cells in each condition using the microfluidic channels. (I) Number of extravasated cells 16 hours post-perfusion of tumor cells (10min), with or without flow, in the microfluidic channels in each condition. In vitro experiment were done at least 3 times, in triplicate. Values in (B-C), (F-G) and (H,I) are mean + SD.